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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Proc Natl Acad Sci U S A. 1992;89(5):1880-4.
Functional assembly of a randomly cleaved protein.
Shiba K, Schimmel P.
(
1542687
)
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J Biol Chem. 1985;260(9):5610-5.
Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.
Tokunaga M, Loranger JM, Chang SY, Regue M, Chang S, Wu HC.
(
2580835
)
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Deletion
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OCR27
(D)
,
OCR27-9
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
ileS
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Related strains
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Strains Search
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Search strains by gene name:
ileS
Search strains by all related name:
ileS b0026 ECK0027 ilvS JW0024
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General information (Go to Linear View: )
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Gene Name
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ileS
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Alternative name
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b0026,ECK0027,ilvS,JW0024
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Location, Length
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22,391 - 25,207
(
+
)
;
0.48
min
;
2817
(bp) ,
938
(aa)
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Product
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isoleucyl-tRNA synthetase
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Operon Name
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ribF-ileS-lspA-fkpB-ispH
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Note
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isoleucine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( ileS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( ileS )
GO:0004822
;
isoleucine-tRNA ligase activity ( ileS )
GO:0005515
;
protein binding ( ileS )
GO:0005524
;
ATP binding ( ileS )
GO:0005737
;
cytoplasm ( ileS )
GO:0006412
;
translation ( ileS )
GO:0006418
;
tRNA aminoacylation for protein translation ( ileS )
GO:0006428
;
isoleucyl-tRNA aminoacylation ( ileS )
GO:0008270
;
zinc ion binding ( ileS )
GO:0016874
;
ligase activity ( ileS )
GO:0046677
;
response to antibiotic ( ileS )
GO:0046872
;
metal ion binding ( ileS )
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PID
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2367096
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EC number
(KEGG Pathway)
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6.1.1.5
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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ileS
(
EG10492
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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SYI_ECOLI
(
P00956
;
P78038; Q59429
)
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- Protein name
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Isoleucyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.5, Isoleucine--tRNA ligase, IleRS
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- Gene name
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Name=ileS; Synonyms=ilvS; OrderedLocusNames=b0026;
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- Comments
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CATALYTIC ACTIVITY:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
COFACTOR:Binds 1 zinc ion per subunit.
SUBUNIT:Monomer.
SUBCELLULAR LOCATION:Cytoplasmic.
MISCELLANEOUS:Strain PS102 is resistant to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase thus inhibiting protein biosynthesis.
SIMILARITY:Belongs to class-I aminoacyl-tRNA synthetase family.
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Entry name(Acc.no)
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Q8FLB7
(
Q8FLB7
)
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- Protein name
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Isoleucyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.5
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- Gene name
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Name=ileS; OrderedLocusNames=c0030;
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- Comments
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SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.
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Entry name(Acc.no)
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Q8XA49
(
Q8XA49
)
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- Protein name
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Isoleucine tRNA synthetase.
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- Synonyms
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- Gene name
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Name=ileS; OrderedLocusNames=z0030;
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- Comments
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SUBCELLULAR LOCATION:Integral membrane protein (By similarity).
SIMILARITY:Belongs to the sugar transporter family.
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MMBR References
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J Biol Chem. 1985;260:5616-5620
Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon.
Kamio, Y., C.-K. Lin, M. Regue, and H. C. Wu.
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J Biol Chem. 1987;262:389-393
Cotranscription of the isoleucyl-tRNA synthetase (ileS) and prolipoprotein signal peptidase(lsp) genes: fine-structure mapping of the lsp internal promoter.
Miller, K. W., and H. C. Wu.
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J Bacteriol. 1984;158(2):632-5.
Mapping of the lipoprotein signal peptidase gene (lsp).
Regue M, Remenick J, Tokunaga M, Mackie GA, Wu HC.
(
6373724
)
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J Bacteriol. 1968;95(5):1672-9.
Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine.
Szentirmai A, Szentirmai M, Umbarger HE.
(
4297021
)
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J Biol Chem. 1985;260:5610-5615
Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.
Tokunaga, M., J. M. Loranger, S.-Y. Chang, M. Regue, S. Chang, and H. C. Wu.
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FEBS Lett. 1984;171(2):245-8.
The gene coding for lipoprotein signal peptidase (lspA) and that for isoleucyl-tRNA synthetase (ileS) constitute a cotranscriptional unit in Escherichia coli.
Yamada H, Kitagawa M, Kawakami M, Mizushima S.
(
6373377
)
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Mol Gen Genet. 1983;192(1-2):10-4.
Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli.
Yamagata H, Taguchi N, Daishima K, Mizushima S.
(
6227793
)
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| Sequences |
Amino acid
FASTA format
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0001 MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT FILHDGPPYA NGSIHIGHSV
0071 NKILKDIIVK SKGLSGYDSP YVPGWDCHGL PIELKVEQEY GKPGEKFTAA EFRAKCREYA ATQVDGQRKD
0141 FIRLGVLGDW SHPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCVD CRSALAEAEV EYYDKTSPSI
0211 DVAFQAVDQD ALKAKFAVSN VNGPISLVIW TTTPWTLPAN RAISIAPDFD YALVQIDGQA VILAKDLVES
0281 VMQRIGVTDY TILGTVKGAE LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG
0351 LETANPVGPD GTYLPGTYPT LDGVNVFKAN DIVVALLQEK GALLHVEKMQ HSYPCCWRHK TPIIFRATPQ
0421 WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVANRP DWCISRQRTW GVPMSLFVHK DTEELHPRTL
0491 ELMEEVAKRV EVDGIQAWWD LDAKEILGDE ADQYVKVPDT LDVWFDSGST HSSVVDVRPE FAGHAADMYL
0561 EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG RKMSKSIGNT VSPQDVMNKL GADILRLWVA
0631 STDYTGEMAV SDEILKRAAD SYRRIRNTAR FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAKAAQEDIL
0701 KAYEAYDFHE VVQRLMRFCS VEMGSFYLDI IKDRQYTAKA DSVARRSCQT ALYHIAEALV RWMAPILSFT
0771 ADEVWGYLPG EREKYVFTGE WYEGLFGLAD SEAMNDAFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV
0841 TLYAEPELSA KLTALGDELR FVLLTSGATV ADYNDAPADA QQSEVLKGLK VALSKAEGEK CPRCWHYTQD
0911 VGKVAEHAEI CGRCVSNVAG DGEKRKFA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GATTGCGCGT GATGAATTAA CCGCCCGCGA
-070 ATTTTTTGGG CTAACAAAAC CGGCTTAAGC CTGTTATGTA ATCAAACCGA AATACGGAAC CGAGAATCTG
0001 atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat ctcgccaagc
0071 gcgaacccgg aatgctggcg cgttggactg atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg
0141 caaaaaaacc ttcattctgc atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt
0211 aacaagattc tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
0281 gctgggactg ccacggtctg ccgatcgagc tgaaagtcga gcaagaatac ggtaagccgg gtgagaaatt
0351 caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca acgcaaagac
0421 tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa actgaagcca
0491 acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg
0561 gtgcgttgac tgccgttctg cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc
0631 gacgttgctt tccaggcagt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
0701 caatctcgct ggtaatctgg accaccacgc cgtggactct gcctgccaac cgcgcaatct ctattgcacc
0771 agatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct ggttgaaagc
0841 gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa aggtgcggag cttgagctgc
0911 tgcgctttac ccatccgttt atgggcttcg acgttccggc aatcctcggc gatcacgtta ccctggatgc
0981 cggtaccggt gccgttcaca ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc
1051 ctggaaaccg ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
1121 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga
1191 gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc gacgccgcag
1261 tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc gtgcagtgga
1331 tcccggactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct gactggtgta tctcccgtca
1401 gcgcacctgg ggtgtaccga tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtaccctt
1471 gaactgatgg aagaagtggc aaaacgcgtt gaagtcgatg gcatccaggc gtggtgggat ctcgatgcga
1541 aagagatcct cggcgacgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc
1611 cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg
1681 gaaggttctg accaacaccg cggctggttc atgtcttccc taatgatctc caccgcgatg aagggtaaag
1751 cgccgtatcg tcaggtactg acccacggct ttaccgtgga tggtcagggc cgcaagatgt ctaaatccat
1821 cggcaatacc gtttcgccgc aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca
1891 tcaaccgact acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
1961 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag atatggtgaa
2031 accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga agacatcctc
2101 aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc gttgagatgg
2171 gttccttcta cctcgacatc atcaaagacc gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag
2241 ctgccagact gcgctatatc acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc
2311 gctgatgaag tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
2381 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg
2451 cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg gctcgctgga agcggcagta
2521 accttgtatg cagaaccgga actgtcggcg aaactgaccg cgctgggcga tgaattacga tttgtcctgt
2591 tgacctccgg cgctaccgtt gcagactata acgacgcacc tgctgatgct cagcagagcg aagtactcaa
2661 agggctgaaa gtcgcgttga gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat
2731 gtcggcaagg tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
2801 aacgtaagtt tgcctgaTGA GTCAATCGAT CTGTTCAACA GGGCTACGCT GGCTGTGGCT GGTGGTAGTC
2871 GTGCTGATTA TCGATCTGGG CAGCAAATAC CTGATCCTCC AGAACTT
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