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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Mol Gen Genet. 1980;177(4):603-5.
A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12.
Bohman K, Isaksson LA.
(
6991867
)
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Deletion
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OCR35-4
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
proS
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Related strains
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Strains Search
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Search strains by gene name:
proS
Search strains by all related name:
proS b0194 drp drpA ECK0194 JW0190
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General information (Go to Linear View: )
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Gene Name
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proS
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Alternative name
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b0194,drp,drpA,ECK0194,JW0190
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Location, Length
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217,057 - 218,775
(
-
)
;
4.68
min
;
1719
(bp) ,
572
(aa)
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Product
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prolyl-tRNA synthetase
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Operon Name
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proS
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Note
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proline tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( proS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( proS )
GO:0004827
;
proline-tRNA ligase activity ( proS )
GO:0005515
;
protein binding ( proS )
GO:0005524
;
ATP binding ( proS )
GO:0005737
;
cytoplasm ( proS )
GO:0006412
;
translation ( proS )
GO:0006418
;
tRNA aminoacylation for protein translation ( proS )
GO:0006433
;
prolyl-tRNA aminoacylation ( proS )
GO:0016874
;
ligase activity ( proS )
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PID
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1786392
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EC number
(KEGG Pathway)
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6.1.1.15
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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proS
(
EG10770
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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SYP_ECOLI
(
P16659
;
P78272; Q59430
)
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- Protein name
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Prolyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.15, Proline--tRNA ligase, ProRS, Global RNA synthesis factor
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- Gene name
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Name=proS; Synonyms=drpA; OrderedLocusNames=b0194;
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- Comments
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CATALYTIC ACTIVITY:ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to class-II aminoacyl-tRNA synthetase family.
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Entry name(Acc.no)
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Q8FKZ4
(
Q8FKZ4
)
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- Protein name
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Prolyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.15
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- Gene name
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Name=proS; OrderedLocusNames=c0235;
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- Comments
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SIMILARITY:Belongs to class-II aminoacyl-tRNA synthetase family.
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Entry name(Acc.no)
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Q8X8W2
(
Q8X8W2
)
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- Protein name
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Proline tRNA synthetase.
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- Synonyms
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- Gene name
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Name=proS; OrderedLocusNames=z0206;
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- Comments
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SIMILARITY:Belongs to class-II aminoacyl-tRNA synthetase family.
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MMBR References
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J Bacteriol. 1985;162(1):117-23.
New gene in Escherichia coli K-12 (drpA): does its product play a role in RNA synthesis?
Lech KF, Lee CH, Isberg RR, Syvanen M.
(
2579939
)
|
Mol Gen Genet. 1980;177(4):603-5.
A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12.
Bohman K, Isaksson LA.
(
6991867
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGVRVL KKVENIVREE MNNAGAIEVS
0071 MPVVQPADLW QESGRWEQYG PELLRFVDRG ERPFVLGPTH EEVITDLIRN ELSSYKQLPL NFYQIQTKFR
0141 DEVRPRFGVM RSREFLMKDA YSFHTSQESL QETYDAMYAA YSKIFSRMGL DFRAVQADTG SIGGSASHEF
0211 QVLAQSGEDD VVFSDTSDYA ANIELAEAIA PKEPRAAATQ EMTLVDTPNA KTIAELVEQF NLPIEKTVKT
0281 LLVKAVEGSS FPQVALLVRG DHELNEVKAE KLPQVASPLT FATEEEIRAV VKAGPGSLGP VNMPIPVVID
0351 RTVAAMSDFA AGANIDGKHY FGINWDRDVA TPEVADIRNV VAGDPSPDGQ GRLLIKRGIE VGHIFQLGTK
0421 YSEALKASVQ GEDGRNQILT MGCYGIGVTR VVAAAIEQNY DERGIVWPDA IAPFQVAILP MNMHKSFRVQ
0491 ELAEKLYSEL RAQGIEVLLD DRKERPGVMF ADMELIGIPH TIVLGDRNLD NDDIEYKYRR NGEKQLIKTG
0561 DIVEYLVKQI KG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tctcttttga catttctttt gcactggtaa
-070 actaaatcac ttttttttgt cccaggctcg ccttgagcct gttctacctt ccaactggaa ccgtaacaac
0001 atgcgtacta gccaatacct gctctccact ctcaaggaga cacctgccga cgccgaggtg atcagccatc
0071 agctgatgct gcgcgccggg atgatccgca agctggcctc cgggttatat acctggctgc cgaccggcgt
0141 gcgcgttctg aaaaaagtcg aaaacatcgt gcgtgaagag atgaacaacg ccggtgcgat cgaggtgtcg
0211 atgccggtgg ttcagccagc cgatttgtgg caagagagtg gtcgttggga acagtacggt ccggaactgc
0281 tgcgttttgt tgaccgtggc gagcgtccgt tcgtactcgg cccaactcat gaagaagtta tcactgacct
0351 gattcgtaac gagcttagct cttacaaaca gctgccgctg aacttctatc agatccagac caagttccgc
0421 gacgaagtgc gtccgcgttt cggcgtcatg cgttcccgcg aattcctgat gaaagatgct tactctttcc
0491 atacttctca ggaatccctg caggaaacct acgatgcaat gtatgcggcc tacagcaaaa tcttcagccg
0561 catggggctg gatttccgcg ccgtacaagc cgacaccggt tctatcggcg gcagcgcctc tcacgaattc
0631 caggtgctgg cgcagagcgg tgaagacgat gtggtcttct ccgacacctc tgactatgca gcgaacattg
0701 aactggcaga agctatcgcg ccgaaagaac cgcgcgctgc tgctacccag gaaatgacgc tggttgatac
0771 gccgaacgcg aaaaccatcg cggaactggt tgaacagttc aatctgccga ttgagaaaac ggttaagact
0841 ctgctggtta aagcggttga aggcagcagc ttcccgcagg ttgcgctgct ggtgcgcggt gatcacgagc
0911 tgaacgaagt taaagcagaa aaactgccgc aggttgcaag cccgctgact ttcgcgaccg aagaagaaat
0981 tcgtgccgtg gttaaagccg gtccgggttc actgggtccg gtaaacatgc cgattccggt ggtgattgac
1051 cgtaccgttg cggcgatgag tgatttcgct gctggtgcta acatcgatgg taaacactac ttcggcatca
1121 actgggatcg cgatgtcgct accccggaag ttgcagatat ccgtaacgtg gtggctggcg atccaagccc
1191 ggatggccag ggtaggctgc tgatcaaacg tggtatcgaa gttggtcaca tcttccagct gggtaccaag
1261 tactccgaag cactgaaagc ctccgtacag ggtgaagatg gccgtaacca aatcctgacg atgggttgct
1331 acggtatcgg ggtaacgcgt gtggtagctg cggcgattga gcagaactac gacgaacgag gcatcgtatg
1401 gcctgacgct atcgcgccgt tccaggtggc gattctgccg atgaacatgc acaaatcctt ccgcgtacaa
1471 gagcttgctg agaaactgta cagcgaactg cgtgcacaag gtatcgaagt gctgctggat gaccgcaaag
1541 agcgtccggg cgtgatgttt gctgatatgg aactgatcgg tattccgcac actattgtgc tgggcgaccg
1611 taacctcgac aacgacgata tcgaatataa atatcgtcgc aacggcgaga aacagttaat taagactggt
1681 gacatcgtcg aatatctggt gaaacagatt aaaggctgat gccagaaagg gtcctgaatt tcagggccct
1751 ttttttacat ggattgtata aaatggataa accaaaggcg tactgccgcc tgtttctccc cagctttct
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