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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
murD
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Related strains
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Strains Search
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Search strains by gene name:
murD
Search strains by all related name:
murD b0088 ECK0089 JW0086
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General information (Go to Linear View: )
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Gene Name
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murD
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Alternative name
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b0088,ECK0089,JW0086
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Location, Length
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97,087 - 98,403
(
+
)
;
2.09
min
;
1317
(bp) ,
438
(aa)
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Product
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UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
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Operon Name
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mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
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GO:0000166
;
nucleotide binding ( murD )
GO:0003824
;
catalytic activity ( murD )
GO:0005488
;
binding ( murD )
GO:0005515
;
protein binding ( murD )
GO:0005524
;
ATP binding ( murD )
GO:0005737
;
cytoplasm ( murD )
GO:0007047
;
cell wall organization and biogenesis ( murD )
GO:0007049
;
cell cycle ( murD )
GO:0008152
;
metabolic process ( murD )
GO:0008360
;
regulation of cell shape ( murD )
GO:0008764
;
UDP-N-acetylmuramoylalanine-D-glutamate ligase activity ( murD )
GO:0009058
;
biosynthetic process ( murD )
GO:0009252
;
peptidoglycan biosynthetic process ( murD )
GO:0009273
;
peptidoglycan-based cell wall biogenesis ( murD )
GO:0016874
;
ligase activity ( murD )
GO:0051301
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cell division ( murD )
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PID
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1786276
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EC number
(KEGG Pathway)
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6.3.2.9
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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murD
(
EG10620
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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MURD_ECOLI
(
P14900
)
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- Protein name
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UDP-N-acetylmuramoylalanine--D-glutamate ligase
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- Synonyms
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EC 6.3.2.9, UDP-N- acetylmuramoyl-L-alanyl-D-glutamate synthetase, D-glutamic acid adding enzyme
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- Gene name
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Name=murD; OrderedLocusNames=b0088;
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MMBR References
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Nucleic Acids Res. 1990;18(4):1058
Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli.
Ikeda M, Wachi M, Ishino F, Matsuhashi M.
(
2179861
)
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J Bacteriol. 1989;171(11):6126-34.
Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J.
(
2681153
)
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Nucleic Acids Res. 1990;18(1):183
Nucleotide sequence of the murD gene encoding the UDP-MurNAc-L-Ala-D-Glu synthetase of Escherichia coli.
Mengin-Lecreulx D, van Heijenoort J.
(
2129548
)
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Eur J Biochem. 1991;202:1169-1176
Overproduction, purification and properties of the uridine diphsophate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli.
Pratviel-Sosa, F., D. Mengin-Lecreulx, and J. van Heijenoort.
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| Sequences |
Amino acid
FASTA format
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0001 MADYQGKNVV IIGLGLTGLS CVDFFLARGV TPRVMDTRMT PPGLDKLPEA VERHTGSLND EWLMAADLIV
0071 ASPGIALAHP SLSAAADAGI EIVGDIELFC REAQAPIVAI TGSNGKSTVT TLVGEMAKAA GVNVGVGGNI
0141 GLPALMLLDD ECELYVLELS SFQLETTSSL QAVAATILNV TEDHMDRYPF GLQQYRAAKL RIYENAKVCV
0211 VNADDALTMP IRGADERCVS FGVNMGDYHL NHQQGETWLR VKGEKVLNVK EMKLSGQHNY TNALAALALA
0281 DAAGLPRASS LKALTTFTGL PHRFEVVLEH NGVRWINDSK ATNVGSTEAA LNGLHVDGTL HLLLGGDGKS
0351 ADFSPLARYL NGDNVRLYCF GRDGAQLAAL RPEVAEQTET MEQAMRLLAP RVQPGDMVLL SPACASLDQF
0421 KNFEQRGNEF ARLAKELG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 AACTGAAAGG CTGGCCGGAA CCGCGCGTCA
-070 TTGTGCGTTT CTGGATTATT TCGCTGATGC TGGTTCTGAT TGGTCTGGCA ACGCTGAAGG TACGTTAATC
0001 atggctgatt atcagggtaa aaatgtcgtc attatcggcc tgggcctcac cgggctttcc tgcgtggact
0071 ttttcctcgc tcgcggtgtg acgccgcgcg ttatggatac gcgtatgaca ccgcctggcc tggataaatt
0141 acccgaagcc gtagaacgcc acacgggcag tctgaatgat gaatggctga tggcggcaga tctgattgtc
0211 gccagtcccg gtattgcact ggcgcatcca tccttaagcg ctgccgctga tgccggaatc gaaatcgttg
0281 gcgatatcga gctgttctgt cgcgaagcac aagcaccgat tgtggcgatt accggttcta acggcaaaag
0351 cacggtcacc acgctagtgg gtgaaatggc gaaagcggcg ggggttaacg ttggtgtggg tggcaatatt
0421 ggcctgcctg cgttgatgct actggatgat gagtgtgaac tgtacgtgct ggaactgtcg agcttccagc
0491 tggaaaccac ctccagctta caggcggtag cagcgaccat tctgaacgtg actgaagatc atatggatcg
0561 ctatccgttt ggtttacaac agtatcgtgc agcaaaactg cgcatttacg aaaacgcgaa agtttgcgtg
0631 gttaatgctg atgatgcctt aacaatgccg attcgcggtg cggatgaacg ctgcgtcagc tttggcgtca
0701 acatgggtga ctatcacctg aatcatcagc agggcgaaac ctggctgcgg gttaaaggcg agaaagtgct
0771 gaatgtgaaa gagatgaaac tttccgggca gcataactac accaatgcgc tggcggcgct ggcgctggca
0841 gatgctgcag ggttaccgcg tgccagcagc ctgaaagcgt taaccacatt cactggtctg ccgcatcgct
0911 ttgaagttgt gctggagcat aacggcgtac gttggattaa cgattcgaaa gcgaccaacg tcggcagtac
0981 ggaagcggcg ctgaatggcc tgcacgtaga cggcacactg catttgttgc tgggtggcga tggtaaatcg
1051 gcggacttta gcccactggc gcgttacctg aatggcgata acgtacgtct gtattgtttc ggtcgtgacg
1121 gcgcgcagct ggcggcgcta cgcccggaag tggcagaaca aaccgaaact atggaacagg cgatgcgctt
1191 gctggctccg cgtgttcagc cgggcgatat ggttctgctc tccccagcct gtgccagcct tgatcagttc
1261 aagaactttg aacaacgagg caatgagttt gcccgtctgg cgaaggagtt aggttgaTGC GTTTATCTCT
1331 CCCTCGCCTG AAAATGCCGC GCCTGCCAGG ATTCAGTATC CTGGTCTGGA TCTCCACGGC GCTAAAGGGC
1401 TGGGTGATGG GCTCGCG
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