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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
|
OCR11
(D)
,
DE(aceA-pgi)33
|
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
aceK
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Related strains
|
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Strains Search
|
Search strains by gene name:
aceK
Search strains by all related name:
aceK b4016 ECK4008 JW3976 o578
|
General information (Go to Linear View: )
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Gene Name
|
aceK
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Alternative name
|
b4016,ECK4008,JW3976,o578
|
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Location, Length
|
4,216,619 - 4,218,355
(
+
)
;
90.88
min
;
1737
(bp) ,
578
(aa)
|
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Product
|
isocitrate dehydrogenase kinase/phosphatase
|
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Operon Name
|
aceBAK
|
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Note
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GO_process: GO:0006097 - glyoxylate cycle; GO_process: GO:0006464 - protein modification process
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Function
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enzyme; Central intermediary metabolism: Glyoxylate bypass
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( aceK )
GO:0003824
;
catalytic activity ( aceK )
GO:0004721
;
phosphoprotein phosphatase activity ( aceK )
GO:0005524
;
ATP binding ( aceK )
GO:0005737
;
cytoplasm ( aceK )
GO:0006006
;
glucose metabolic process ( aceK )
GO:0006097
;
glyoxylate cycle ( aceK )
GO:0006099
;
tricarboxylic acid cycle ( aceK )
GO:0008772
;
[isocitrate dehydrogenase (NADP+)] kinase activity ( aceK )
GO:0016301
;
kinase activity ( aceK )
GO:0016740
;
transferase activity ( aceK )
GO:0016787
;
hydrolase activity ( aceK )
GO:0016788
;
hydrolase activity, acting on ester bonds ( aceK )
GO:0016791
;
phosphoric monoester hydrolase activity ( aceK )
|
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PID
|
1790446
|
EC number
(KEGG Pathway)
|
2.7.11.5
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
aceK
(
EG10026
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
ACEK_ECOLI
(
P11071
;
P11070
)
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- Protein name
|
Isocitrate dehydrogenase kinase/phosphatase
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- Synonyms
|
EC 2.7.1.116, EC 3.1.3.-, IDH kinase/phosphatase, IDHK/P
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- Gene name
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Name=aceK; OrderedLocusNames=b4016;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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aceK
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Isocitrate dehydrogenase kinase/phosphatase
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PSI-BLAST
|
Isocitrate dehydrogenase kinase/phosphatase
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
bifunctional isocitrate dehydrogenase kinase/phosphatase protein [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
BPD_TRANSP_INN_MEMBR
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MMBR References
|
Gene. 1991;97(1):149-50.
Primary structure of the intergenic region between aceK and iclR in the Escherichia coli chromosome.
Galinier A, Bleicher F, Negre D, Perriere G, Duclos B, Cozzone AJ, Cortay JC.
(
1995429
)
|
J Bacteriol. 1991;173(5):1801-6.
Isocitrate dehydrogenase kinase/phosphatase: aceK alleles that express kinase but not phosphatase activity.
Ikeda T, LaPorte DC.
(
1847910
)
|
J Bacteriol. 1988;170(6):2763-9.
Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.
Klumpp DJ, Plank DW, Bowdin LJ, Stueland CS, Chung T, LaPorte DC.
(
2836370
)
|
J Biol Chem. 1985;260:10563-10568
Compensatory phosphorylation of isocitrate dehydrogenase. A mechanism for adaption to the intracellular environment.
LaPorte, D. C., P. E. Thorsness, and D. E. Koshland.
|
J Biol Chem. 1985;260:15291-15297
A single gene codes for the kinase and phosphatase which regulate isocitrate dehydrogenase.
LaPorte, D. C., and T. Chung.
|
J Bacteriol. 1988;170(1):386-92.
Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map.
Chung T, Klumpp DJ, LaPorte DC.
(
2826399
)
|
J Bacteriol. 1993;175:4572-4575
Relative expression of the products of the glyoxylate bypass operon: contributions of transcription and translation.
Chung, T., E. Resnik, C. S. Stueland, and D. C. LaPorte.
|
J Bacteriol. 1988;170(1):89-97.
Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli.
Cortay JC, Bleicher F, Rieul C, Reeves HC, Cozzone AJ, Klumpp DJ.
(
2826408
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR IHLYDHHVGL VVEQLRCITN
0071 GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA
0141 KDFHPDHGWE SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL FYRNKAAWLV
0211 GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT
0281 AELYMAIGCQ KHAKTESYRE YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA
0351 AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK ITDLGEQIVI RHLYIERRMV
0421 PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI
0491 PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN RIREGHVEDV
0561 YAYRRRQRFS VRYGEMLF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCTTGCGCGT CTTATCATGC CTACAGCCGT
-070 TGCCGAACGT AGGCTGGATA AGGCGTTTAC GCCGCATCCG GCAATTCTCT GCTCCTGATG AGGGCGCTAA
0001 atgccgcgtg gcctggaatt attgattgct caaaccattt tgcaaggctt cgatgctcag tatggtcgat
0071 tcctcgaagt gacctccggt gcgcagcagc gtttcgaaca ggccgactgg catgctgtcc agcaggcgat
0141 gaaaaaccgt atccatcttt acgatcatca cgttggtctg gtcgtggagc aactgcgctg cattactaac
0211 ggccaaagta cggacgcggc atttttacta cgtgttaaag agcattacac ccggctgttg ccggattacc
0281 cgcgcttcga gattgcggag agctttttta actccgtgta ctgtcggtta tttgaccacc gctcgcttac
0351 tcccgagcgg ctttttatct ttagctctca gccagagcgc cgctttcgta ccattccccg cccgctggcg
0421 aaagactttc accccgatca cggctgggaa tctctactga tgcgcgttat cagcgaccta ccgctgcgcc
0491 tgcgctggca gaataaaagc cgtgacatcc attacattat tcgccatctg acggaaacgc tggggacaga
0561 caacctcgcg gaaagtcatt tacaggtggc gaacgaactg ttttaccgca ataaagccgc ctggctggta
0631 ggcaaactga tcacaccttc cggcacattg ccatttttgc tgccgatcca ccagacggac gacggcgagt
0701 tatttattga tacctgcctg acgacgaccg ccgaagcgag cattgttttt ggctttgcgc gttcttattt
0771 tatggtttat gcgccgctgc ccgcagcgct ggtcgagtgg ctacgggaaa ttctgccagg taaaaccacc
0841 gctgaattgt atatggctat cggctgccag aagcacgcca aaaccgaaag ctaccgcgaa tatctcgttt
0911 atctacaggg ctgtaatgag cagttcattg aagcgccggg tattcgtgga atggtgatgt tggtgtttac
0981 gctgccgggc tttgatcggg tattcaaagt catcaaagac aggttcgcgc cgcagaaaga gatgtctgcc
1051 gctcacgttc gtgcctgcta tcaactggtg aaagagcacg atcgcgtggg ccgaatggcg gacacccagg
1121 agtttgaaaa ctttgtgctg gagaagcggc atatttcccc ggcattaatg gaattactgc ttcaggaagc
1191 agcggaaaaa atcaccgatc tcggcgaaca aattgtgatt cgccatcttt atattgagcg gcggatggtg
1261 ccgctcaata tctggctgga acaagtggaa ggtcagcagt tgcgcgacgc cattgaagaa tacggtaacg
1331 ctattcgcca gcttgccgct gctaacattt tccctggcga catgctgttt aaaaacttcg gtgtcacccg
1401 tcacgggcgt gtggtttttt atgattacga tgaaatttgc tacatgacgg aagtgaattt ccgcgacatc
1471 ccgccgccgc gctatccgga agacgaactt gccagcgaac cgtggtacag cgtctcgccg ggcgatgttt
1541 tcccggaaga gtttcgccac tggctatgcg ccgacccgcg tattggtccg ctgtttgaag agatgcacgc
1611 cgacctgttc cgcgctgatt actggcgcgc actacaaaac cgcatacgtg aagggcatgt ggaagatgtt
1681 tatgcgtatc ggcgcaggca aagatttagc gtacggtatg gggagatgct tttttgaGTA AAGCTTCCAT
1751 ATAATTTTTC TCCGCAATGT ATCGAGGGTT ATCCGTAAAG CCAAAGCTTT CAGCCATCTT ATTTAATGTA
1821 TTAAGGATTA ATTCAGC
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