MG1655
W3110
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Gene Report : kefC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  kefC
Related strains
     Strains Search Search strains by gene name:  kefC   Search strains by all related name:  kefC b0047 ECK0048 JW0046 trkC

General information  (Go to Linear View:)
 Gene Name kefC  
 Alternative name b0047,ECK0048,JW0046,trkC  
 Location, Length 47,769 - 49,631 (  +  ) ;   1.03 min ; 1863 (bp) ,   620 (aa) Go to Linear View
 Product potassium:proton antiporter  
 Operon Name kefFC  
 Note K+ efflux antiporter, glutathione-regulated; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_function: GO:0015386 - potassium:hydrogen antiporter activity  
 Function transport; Transport of small molecules: Cations  
 Gene Ontology GO:0003824 ; catalytic activity ( kefC )
GO:0005488 ; binding ( kefC )
GO:0005886 ; plasma membrane ( kefC )
GO:0006810 ; transport ( kefC )
GO:0006811 ; ion transport ( kefC )
GO:0006812 ; cation transport ( kefC )
GO:0006813 ; potassium ion transport ( kefC )
GO:0006885 ; regulation of pH ( kefC )
GO:0008152 ; metabolic process ( kefC )
GO:0008324 ; cation transmembrane transporter activity ( kefC )
GO:0015297 ; antiporter activity ( kefC )
GO:0015299 ; solute:hydrogen antiporter activity ( kefC )
GO:0015503 ; glutathione-regulated potassium exporter activity ( kefC )
GO:0016020 ; membrane ( kefC )
GO:0016021 ; integral to membrane ( kefC )
GO:0030955 ; potassium ion binding ( kefC )
 PID 1786232  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) KEFC_ECOLI ( P03819 )
    -  Protein name Glutathione-regulated potassium-efflux system protein kefC (K  
    -  Synonyms +)/H(+) antiporter  
    -  Gene name Name=kefC; Synonyms=trkC; OrderedLocusNames=b0047;  

 Linear View (Whole Mode)
View Location
   35.0  –  60.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP kefC (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Mthk At 3.3 A  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glutathione-regulated potassium-efflux system protein kefC  
         PSI-BLAST Adenosylhomocysteinase
    nr     (database updated : 2007.02.20 )
         BLAST glutathione-regulated potassium-efflux system protein [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    LEUCINE_ZIPPER     

Other Cross-Reference
    COG COG0475P  COG1226P 
    EcoCyc kefC 

MMBR References
J Bacteriol. 1998;180:1030-1036
Importance of RpoS and Dps in survival of exposure of both exponential- and stationary-phase Escherichia coli cells to the electrophile N-ethylmaleimide.
Ferguson, G. P., R. I. Creighton, Y. Nikolaev, and I. R. Booth.
J Bacteriol. 1987;169(8):3743-9.
Evidence for multiple K+ export systems in Escherichia coli.
Bakker EP, Booth IR, Dinnbier U, Epstein W, Gajewska A. ( 3301813 )
J Biol Chem. 1997;272:24942-24947
Mutations in the glutathione-gated KefC K+ efflux system of Escherichia coli that cause constitutive activation.
Miller, S., R. M. Douglas, P. Carter, and I. R. Booth.
Mol Microbiol. 1991;5(3):607-16.
The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli.
Munro AW, Ritchie GY, Lamb AJ, Douglas RM, Booth IR. ( 2046548 )

Sequences
Amino acid
FASTA format 
0001 MDSHTLIQAL IYLGSAALIV PIAVRLGLGS VLGYLIAGCI IGPWGLRLVT DAESILHFAE IGVVLMLFII 
0071 GLELDPQRLW KLRAAVFGCG ALQMVICGGL LGLFCMLLGL RWQVAELIGM TLALSSTAIA MQAMNERNLM 
0141 VTQMGRSAFA VLLFQDIAAI PLVAMIPLLA TSSASTTMGA FALSALKVAG ALVLVVLLGR YVTRPALRFV 
0211 ARSGLREVFS AVALFLVFGF GLLLEEVGLS MAMGAFLAGV LLASSEYRHA LESDIEPFKG LLLGLFFIGV 
0281 GMSIDFGTLL ENPLRIVILL LGFLIIKIAM LWLIARPLQV PNKQRRWFAV LLGQGSEFAF VVFGAAQMAN 
0351 VLEPEWAKSL TLAVALSMAA TPILLVILNR LEQSSTEEAR EADEIDEEQP RVIIAGFGRF GQITGRLLLS 
0421 SGVKMVVLDH DPDHIETLRK FGMKVFYGDA TRMDLLESAG AAKAEVLINA IDDPQTNLQL TEMVKEHFPH 
0491 LQIIARARDV DHYIRLRQAG VEKPERETFE GALKTGRLAL ESLGLGPYEA RERADVFRRF NIQMVEEMAM 
0561 VENDTKARAA VYKRTSAMLS EIITEDREHL SLIQRHGWQG TEEGKHTGNM ADEPETKPSS
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             CCGTTTGCCA TGCACTGCAC CTTTATTTGT 
-070 GACGACGAAA CCCTCGAAGG GCAGGCGCGT CACTATAAGC AACGTCTGCT GGAATGGCAG GAGGCCCATC 
0001 atggatagcc atacgctgat tcaggcgctg atttatctcg gttcggcagc gctgattgta cccattgcgg 
0071 tacgtcttgg tctgggatcg gtacttggct acctgatcgc cggctgcatt attggcccgt gggggctgcg 
0141 actggtgacc gatgccgaat ctattctgca ctttgccgag attggggtgg tgctgatgct gtttattatc 
0211 ggcctcgaac tcgatccaca aaggctgtgg aagctgcgtg cggcagtgtt cggctgtggc gcattgcaga 
0281 tggtgatttg cggcggcctg ctggggctgt tctgcatgtt acttgggctg cgctggcagg tcgcggaatt 
0351 gatcggcatg acgctggcgc tctcctctac ggcgattgcc atgcaggcga tgaatgaacg caatctgatg 
0421 gtgacgcaaa tgggtcgcag tgcctttgcg gtgctgctgt tccaggatat cgcggcgatc ccgctggtgg 
0491 cgatgattcc gctactggca acgagcagtg cctcgacgac gatgggcgca tttgctctct cggcgttaaa 
0561 agtggcgggt gcgctggtgc tggtggtatt gctggggcgc tatgtcacgc gtccggcgct gcgttttgta 
0631 gcccgctctg gcttgcggga agtgtttagt gccgtggcgt tattcctcgt gtttggcttt ggtttgctgc 
0701 tggaagaggt cggcttgtcg atggcgatgg gcgcgtttct ggcgggcgta ctgctggcaa gctcggaata 
0771 ccgtcatgcg ctggagagcg atatcgaacc atttaaaggt ttgctgttgg ggctgttttt catcggtgtt 
0841 ggcatgtcga tagactttgg cacgctgctt gaaaacccat tgcgcattgt cattttgctg ctcggtttcc 
0911 tcatcatcaa aatcgccatg ctgtggctga ttgcccgacc gttgcaagtg ccaaataaac agcgtcgttg 
0981 gtttgcggtg ttgttagggc agggcagtga gtttgccttt gtggtatttg gcgcggcgca gatggcgaat 
1051 gtgctggagc cggagtgggc gaaatcgctg accctggcgg tggcgctgtc gatggcagca acgccgattc 
1121 tgctggtgat cctcaatcgc cttgagcaat cttctactga ggaagcgcgt gaagccgatg agatcgacga 
1191 agaacagccg cgcgtgatta tcgccggatt cggtcgtttt gggcagatta ccggacgttt actgctctcc 
1261 agcggggtga aaatggtggt actcgatcac gatccggacc atatcgaaac cttgcgtaaa tttggtatga 
1331 aagtgtttta tggcgatgcc acgcggatgg atttactgga atctgccgga gcggcgaaag cggaagtgct 
1401 gattaacgcc atcgacgatc cgcaaaccaa cctgcaactg acagagatgg tgaaagaaca tttcccgcat 
1471 ttgcagatta ttgcccgcgc ccgcgatgtc gaccactaca ttcgtttgcg tcaggcaggc gttgaaaagc 
1541 cggagcgtga aaccttcgaa ggtgcgctga aaaccgggcg tctggcactg gaaagtttag gtctggggcc 
1611 gtatgaagcg cgagaacgtg ccgatgtgtt ccgccgcttt aatattcaga tggtggaaga gatggcaatg 
1681 gttgagaacg acaccaaagc ccgcgcggcg gtctataaac gcaccagcgc gatgttaagt gagatcatta 
1751 ccgaggaccg cgaacatctg tcattaattc aacgacatgg ctggcaggga accgaagaag gtaaacatac 
1821 cggcaacatg gcggatgaac cggaaacgaa accctcatcc taaTAAAGAG TGACGTAAAT CACACTTTAC 
1891 AGCTAACTGT TTGTTTTTGT TTCATTGTAA TGCGGCGAGT CCAGGGAGAG AGCGTGGACT CGCCAGCAGA 
1961 ATA