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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(xthA-pncA)90
,
LD3-17-1
(LD)
,
OCL84,85-1
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
selD
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Related strains
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Strains Search
|
Search strains by gene name:
selD
Search strains by all related name:
selD b1764 ECK1762 fdhB JW1753
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General information (Go to Linear View: )
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Gene Name
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selD
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Alternative name
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b1764,ECK1762,fdhB,JW1753
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Location, Length
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1,844,989 - 1,846,032
(
-
)
;
39.77
min
;
1044
(bp) ,
347
(aa)
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Product
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selenophosphate synthase
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Operon Name
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ydjA-selD-topB
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Note
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selenophosphate synthase, H(2)Se added to acrylyl-tRNA; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( selD )
GO:0000287
;
magnesium ion binding ( selD )
GO:0003824
;
catalytic activity ( selD )
GO:0004756
;
selenide, water dikinase activity ( selD )
GO:0005524
;
ATP binding ( selD )
GO:0008430
;
selenium binding ( selD )
GO:0016301
;
kinase activity ( selD )
GO:0016740
;
transferase activity ( selD )
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PID
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1788062
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EC number
(KEGG Pathway)
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2.7.9.3
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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SELD_ECOLI
(
P16456
;
P78172
)
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- Protein name
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Selenide,water dikinase
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- Synonyms
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EC 2.7.9.3, Selenophosphate synthetase, Selenium donor protein
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- Gene name
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Name=selD; Synonyms=fdhB; OrderedLocusNames=b1764;
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MMBR References
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Eur J Biochem. 1992;206:767-773
Selenoprotein synthesis in E. coli. Purification and characterisation of the enzyme catalysing selenium activation.
Ehrenreich, A., K. Forchhammer, P. Tormay, B. Veprek, and A. Bock.
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Nature. 1989;342(6248):453-6.
Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein.
Forchhammer K, Leinfelder W, Bock A.
(
2531290
)
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J Biol Chem. 1992;267:19650-19654
Escherichia coli mutant SELD enzymes. The cysteine 17 residue is essential for selenophosphate formation from ATP and selenide.
Kim, I. V., Z. Veres, and T. C. Stadtman.
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Proc Natl Acad Sci U S A. 1990;87:543-547
In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product.
Leinfelder, W., K. Forchhammer, B. Veprek, E. Zehelein, and A. Bock.
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J Bacteriol. 1991;173(16):4983-93.
Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme.
Sawers G, Heider J, Zehelein E, Bock A.
(
1650339
)
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Biofactors. 1989;2(1):35-44.
Biochemical and genetic analysis of Salmonella typhimurium and Escherichia coli mutants defective in specific incorporation of selenium into formate dehydrogenase and tRNAs.
Stadtman TC, Davis JN, Zehelein E, Bock A.
(
2679652
)
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| Sequences |
Amino acid
FASTA format
|
0001 MSENSIRLTQ YSHGAGCGCK ISPKVLETIL HSEQAKFVDP NLLVGNETRD DAAVYDLGNG TSVISTTDFF
0071 MPIVDNPFDF GRIAATNAIS DIFAMGGKPI MAIAILGWPI NKLSPEIARE VTEGGRYACR QAGIALAGGH
0141 SIDAPEPIFG LAVTGIVPTE RVKKNSTAQA GCKLFLTKPL GIGVLTTAEK KSLLKPEHQG LATEVMCRMN
0211 IAGASFANIE GVKAMTDVTG FGLLGHLSEM CQGAGVQARV DYEAIPKLPG VEEYIKLGAV PGGTERNFAS
0281 YGHLMGEMPR EVRDLLCDPQ TSGGLLLAVM PEAENEVKAT AAEFGIELTA IGELVPARGG RAMVEIR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gtctggattg tgagcaaagg cgcaggaatt
-070 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca ggagatgtcc
0001 atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg cggctgtaaa atttccccaa
0071 aagtgttgga aaccatcctg catagtgagc aggcgaagtt tgttgatccg aatttgcttg tgggtaatga
0141 aacccgcgac gatgcggcgg tgtacgatct gggcaatggc accagcgtta tcagtaccac cgacttcttt
0211 atgccgatcg ttgataatcc tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg
0281 cgatgggggg caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccagaaat
0351 tgcccgcgaa gtgaccgaag gtggacgcta tgcatgtcgt caggcgggta ttgcgctggc tggcggtcac
0421 tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt accgaccgag cgggtgaaga
0491 aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac gaaaccgctg gggatcggcg ttcttaccac
0561 ggctgagaaa aaatcactgt tgaaaccaga acatcaggga ctggcgacgg aagtgatgtg ccggatgaac
0631 atcgcaggcg cgtcctttgc caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc
0701 tgggccactt gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
0771 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa ctttgccagc
0841 tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg tgatccgcaa acttctggcg
0911 gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt caaagctaca gccgccgagt ttggcattga
0981 actgacggca attggcgaac tggtgccagc gcgcggcggt cgtgccatgg ttgagattcg ttaattcaat
1051 gcggttgttt attgccgaaa aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg
1121 aaaggcgatg gctttatcga gtgc
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