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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1987;169(12):5408-15.
Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli.
Beall B, Lutkenhaus J.
(
2824434
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lpxC
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Related strains
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Strains Search
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Search strains by gene name:
lpxC
Search strains by all related name:
lpxC asmB b0096 ECK0097 envA JW0094
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General information (Go to Linear View: )
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Gene Name
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lpxC
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Alternative name
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asmB,b0096,ECK0097,envA,JW0094
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Location, Length
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106,557 - 107,474
(
+
)
;
2.30
min
;
918
(bp) ,
305
(aa)
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Product
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UDP-3-O-acyl N-acetylglucosamine deacetylase
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Operon Name
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mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
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Note
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UDP-3-O-acyl N-acetylglucosamine deacetylase; lipid A biosynthesis; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009245 - lipid A biosynthetic process
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Function
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enzyme; Surface polysaccharides & antigens
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Gene Ontology
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GO:0008610
;
lipid biosynthetic process ( lpxC )
GO:0008759
;
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity ( lpxC )
GO:0009245
;
lipid A biosynthetic process ( lpxC )
GO:0016787
;
hydrolase activity ( lpxC )
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PID
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1786285
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EC number
(KEGG Pathway)
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3.5.1.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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lpxC
(
EG10265
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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LPXC_ECOLI
(
P07652
)
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- Protein name
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UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase
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- Synonyms
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EC 3.5.1.-, UDP-3-O-acyl-GlcNAc deacetylase, envA protein
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- Gene name
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OrderedLocusNames=b0096, c0114, z0106, ECs0100, SF0093, S0095;
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- Comments
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FUNCTION:Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
CATALYTIC ACTIVITY:UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine + H(2)O = UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine + acetate.
PATHWAY:Lipid A biosynthesis; second step.
SIMILARITY:Belongs to the lpxC family.
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MMBR References
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J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M.
(
2553679
)
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J Bacteriol. 1996;178:5138-5143
asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC).
Kloser, A. W., M. W. Laird, and R. Misra.
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J Bacteriol. 1987;169(12):5408-15.
Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli.
Beall B, Lutkenhaus J.
(
2824434
)
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J Bacteriol. 1980;142(2):615-20.
Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ).
Lutkenhaus JF, Wolf-Watz H, Donachie WD.
(
6991482
)
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J Bacteriol. 1980;143(3):1281-8.
Determination of transcriptional units and gene products from the ftsA region of Escherichia coli.
Lutkenhaus JF, Wu HC.
(
6447690
)
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Curr Microbiol. 1980;4:51-55.
Mutations affecting the regulation of gamma-aminobutyrate utilization in Escherichia coli K-12.
Metzer, E., and Y. S. Halpern.
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J Bacteriol. 1987;169(9):4163-70.
Cluster of genes controlling synthesis and activation of 2,3-dihydroxybenzoic acid in production of enterobactin in Escherichia coli.
Nahlik MS, Fleming TP, McIntosh MA.
(
3040680
)
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Microbiol. Rev. 1993;57:655-682
Genetics of lipopolysaccharide biosynthesis in enteric bacteria.
Schnaitman, C. A., and J. D. Klena.
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J Biol Chem. 1996;271:258-263
Regulation of UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase in Escherichia coli. The second enzymatic step of lipid a biosynthesis.
Sorensen, P. G., J. Lutkenhaus, K. Young, S. S. Eveland, M. S. Anderson, and C. R. Raetz.
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J Bacteriol. 1984;160(2):724-32.
Transcriptional organization within an Escherichia coli cell division gene cluster: direction of transcription of the cell separation gene envA.
Sullivan NF, Donachie WD.
(
6094489
)
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J Biol Chem. 1995;270:30384-30391
The envA permeability/cell division gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase.
Young, K., L. L. Silver, D. Bramhill, P. Cameron, S. S. Eveland, C. R. Raetz, S. A. Hyland, and M. S. Anderson.
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| Sequences |
Amino acid
FASTA format
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0001 MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT MLCTCLVNEH
0071 DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI DELNCAKKFV RIKETVRVED
0141 GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD AFMRQISRAR TFGFMRDIEY LQSRGLCLGG
0211 SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE
0281 AWEYVTFQDD AELPLAFKAP SAVLA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GAATTGACTG GAATTTGGGT TTCGAGGCTC
-070 TTTGTGCTAA ACTGGCCCGC CGAATGTATA GTACACTTCG GTTGGATAGG TAATTTGGCG AGATAATACG
0001 atgatcaaac aaaggacact taaacgtatc gttcaggcga cgggtgtcgg tttacatacc ggcaagaaag
0071 tcaccctgac gttacgccct gcgccggcca acaccggggt catctatcgt cgcaccgact tgaatccacc
0141 ggtagatttc ccggccgatg ccaaatctgt gcgtgatacc atgctctgta cgtgtctggt caacgagcat
0211 gatgtacgga tttcaaccgt agagcacctc aatgctgctc tcgcgggctt gggcatcgat aacattgtta
0281 tcgaagttaa cgcgccggaa atcccgatca tggacggcag cgccgctccg tttgtatacc tgctgcttga
0351 cgccggtatc gacgagttga actgcgccaa aaaatttgtt cgcatcaaag agactgttcg tgtcgaagat
0421 ggcgataagt gggctgaatt taagccgtac aatggttttt cgctggattt caccatcgat tttaaccatc
0491 cggctattga ttccagcaac cagcgctatg cgatgaactt ctccgctgat gcgtttatgc gccagatcag
0561 ccgtgcgcgt acgttcggtt tcatgcgtga tatcgaatat ctgcagtccc gtggtttgtg cctgggcggc
0631 agcttcgatt gtgccatcgt tgttgacgat tatcgcgtac tgaacgaaga cggcctgcgt tttgaagacg
0701 aatttgtgcg tcacaaaatg ctcgatgcga tcggtgactt gttcatgtgt ggtcacaata ttattggtgc
0771 atttaccgct tataaatccg gtcatgcact gaataacaaa ctgctgcagg ctgtcctggc gaaacaggaa
0841 gcctgggaat atgtgacctt ccaggacgac gcagaactgc cgttggcctt caaagcgcct tcagctgtac
0911 tggcataaCG ACATTTATAC TGTCGTATAA AATTCGACTG GCAAATCTGG CACTCTCTCC GGCCAGGTGA
0981 ACCAGTCGTT TTTTTTTGAA TTTTATAAGA GCTATAAA
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