MG1655
W3110
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Gene Report : galK
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A. ( 2404280 )
     Deletion 15 (LD)  ,  DE(pgl-grxA)502  ,  DE(nadA-galE)35  ,  15+dps (LD)  ,  OCR48,49-4 (D)  ,  OCR48,50-8 (LD 15D::Cm) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  galK
Related strains
     Strains Search Search strains by gene name:  galK   Search strains by all related name:  galK b0757 ECK0746 galA JW0740

General information  (Go to Linear View:)
 Gene Name galK  
 Alternative name b0757,ECK0746,galA,JW0740  
 Location, Length 788,054 - 789,202 (  -  ) ;   16.99 min ; 1149 (bp) ,   382 (aa) Go to Linear View
 Product galactokinase  
 Operon Name galETKM  
 Note GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0006012 - galactose metabolic process; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009269 - response to desiccation  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0000166 ; nucleotide binding ( galK )
GO:0004335 ; galactokinase activity ( galK )
GO:0005524 ; ATP binding ( galK )
GO:0005737 ; cytoplasm ( galK )
GO:0005975 ; carbohydrate metabolic process ( galK )
GO:0006012 ; galactose metabolic process ( galK )
GO:0008152 ; metabolic process ( galK )
GO:0016301 ; kinase activity ( galK )
GO:0016310 ; phosphorylation ( galK )
GO:0016740 ; transferase activity ( galK )
GO:0016773 ; phosphotransferase activity, alcohol group as acceptor ( galK )
GO:0046835 ; carbohydrate phosphorylation ( galK )
 PID 1786972  
 EC number
  (KEGG Pathway)   		2.7.1.6  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene galK ( EG10363 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) GAL1_ECOLI ( P06976 )
    -  Protein name Galactokinase  
    -  Synonyms EC 2.7.1.6, Galactose kinase  
    -  Gene name Name=galK; Synonyms=galA; OrderedLocusNames=b0757, z0927, ECs0785;  
    -  Comments
  • CATALYTIC ACTIVITY:ATP + D-galactose = ADP + D-galactose 1-phosphate.
  • PATHWAY:Galactose metabolism; first step.
  • SUBCELLULAR LOCATION:Cytoplasmic (Potential).
  • SIMILARITY:Belongs to the GHMP kinase family. GalK subfamily.  
    •   botton Entry name(Acc.no) GAL1_ECOL6 ( Q8FJS1 )
        -  Protein name Galactokinase  
        -  Synonyms EC 2.7.1.6, Galactose kinase  
        -  Gene name Name=galK; OrderedLocusNames=c0833;  
        -  Comments
  • CATALYTIC ACTIVITY:ATP + D-galactose = ADP + D-galactose 1-phosphate.
  • PATHWAY:Galactose metabolism; first step.
  • SUBCELLULAR LOCATION:Cytoplasmic (Potential).
  • SIMILARITY:Belongs to the GHMP kinase family. GalK subfamily.  

    •  Linear View (Whole Mode)
    View Location
       775.0  –  800.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP galK (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of Galactokinase From Pyrococcus Horikoshi  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Galactokinase (Galactose kinase)  
             PSI-BLAST Galactokinase
        nr     (database updated : 2007.02.20 )
             BLAST galactokinase [Escherichia coli O157:H7 str. Sakai]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    PHOSPHOPANTETHEINE    GALACTOKINASE    GHMP_KINASES_ATP     

    Other Cross-Reference
        COG COG0153G 
        EcoCyc galK 

    MMBR References
    J Biol Chem. 1974;249:143-148
    Triornithine-resistant strains of Escherichia coli. Isolation, definition, and genetic studies.
    Barak, Z., and C. Gilvarg.
    Symp. Soc. Gen. Microbiol. 1960;10:115-131.
    Genetic and functional aspects of galactose metabolism in Escherichia coli K-12.
    Lederberg, E. M.
    Mol Gen Genet. 1968;102(1):79-88.
    Negative control of the galactose operon in E. coli.
    Saedler H, Gullon A, Fiethen L, Starlinger P. ( 4883532 )
    Proc Natl Acad Sci U S A. 1963;50:463-9.
    INTERFERENCE WITH GLYCEROKINASE INDUCTION IN MUTANTS OF E. COLI ACCUMULATING GAL-1-P.
    Sundararajan TA. ( 14067091 )
    Mol Microbiol. 1993;10(2):245-51.
    The galactose regulon of Escherichia coli.
    Weickert MJ, Adhya S. ( 7934815 )
    Proc Natl Acad Sci U S A. 1959;45:1786-1791.
    Hereditary defects in galactose metabolism in Escherichia coli mutants. II. Galactose-induced sensitivity.
    Yarmolinsky, M., H. Wiesmeyer, H. M. Kalckar, and E. Jordan.
    GenBank submission X02306. 1995

    Debouck, C.
    Nucleic Acids Res. 1985;13:1841-1853
    Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon.
    Debouck, C., A. Riccio, D. Schumperli, K. McKenney, J. Jeffers, C. Hughes, M. Rosenberg, M. Heusterspreute, and J. Davison.

    Sequences
    Amino acid
    FASTA format 
    0001 MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD DRKVRVMAAD 
0071 YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV 
0141 LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS MPKGVAVVII 
0211 NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS 
0281 ALEQGDLKRM GELMAESHAS MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP 
0351 AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             aatgctggca gagacccagc gagacctgac 
-070 cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa 
0001 atgagtctga aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc 
0071 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc tgccctgcgc 
0141 gattgattat caaaccgtga tcagttgtgc accacgcgat gaccgtaaag ttcgcgtgat ggcagccgat 
0211 tatgaaaatc agctcgacga gttttccctc gatgcgccca ttgtcgcaca tgaaaactat caatgggcta 
0281 actacgttcg tggcgtggtg aaacatctgc aactgcgtaa caacagcttc ggcggcgtgg acatggtgat 
0351 cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta 
0421 ttgcagcagc tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc 
0491 agtttgtagg ctgtaactgc gggatcatgg atcagctaat ttccgcgctc ggcaagaaag atcatgcctt 
0561 gctgatcgat tgccgctcac tggggaccaa agcagtttcc atgcccaaag gtgtggctgt cgtcatcatc 
0631 aacagtaact tcaaacgtac cctggttggc agcgaataca acacccgtcg tgaacagtgc gaaaccggtg 
0701 cgcgtttctt ccagcagcca gccctgcgtg atgtcaccat tgaagagttc aacgctgttg cgcatgaact 
0771 ggacccgatc gtggcaaaac gcgtgcgtca tatactgact gaaaacgccc gcaccgttga agctgccagc 
0841 gcgctggagc aaggcgacct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg 
0911 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg 
0981 tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga tcccggaaga gctggtgcct 
1051 gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta 
1121 aaccatcaca aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga 
1191 ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc tgatggactg gggtgcgac