|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
J Bacteriol. 1978;136(3):1143-58.
Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.
Iwaya M, Goldman R, Tipper DJ, Feingold B, Strominger JL.
(
363690
)
|
|
|
Deletion
|
OCR46
(D)
,
OCR46-10
(D)
,
OCR46-11
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
mrdB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
mrdB
Search strains by all related name:
mrdB b0634 ECK0627 JW0629 rodA
|
General information (Go to Linear View: )
|
|
Gene Name
|
mrdB
|
|
Alternative name
|
b0634,ECK0627,JW0629,rodA
|
|
Location, Length
|
664,424 - 665,536
(
-
)
;
14.32
min
;
1113
(bp) ,
370
(aa)
|
|
Product
|
cell wall shape-determining protein
|
|
Operon Name
|
ybeB-rlmH-mrdAB-rlpA
|
|
Note
|
rod shape-determining membrane protein; sensitivity to radiation and drugs; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
|
|
Function
|
phenotype; Cell division
|
|
Gene Ontology
|
GO:0005886
;
plasma membrane ( mrdB )
GO:0007049
;
cell cycle ( mrdB )
GO:0008360
;
regulation of cell shape ( mrdB )
GO:0009276
;
1-2nm peptidoglycan-based cell wall ( mrdB )
GO:0016020
;
membrane ( mrdB )
GO:0016021
;
integral to membrane ( mrdB )
|
|
PID
|
1786853
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
RODA_ECOLI
(
P15035
;
P13409
)
|
|
- Protein name
|
Rod shape-determining protein rodA.
|
|
- Synonyms
|
|
|
- Gene name
|
OrderedLocusNames=b0634, z0780, ECs0672, SF0647, S0669;
|
|
- Comments
|
FUNCTION:This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
SIMILARITY:Belongs to the ftsW/rodA/spoVE family.
|
|
Entry name(Acc.no)
|
Q8FJZ3
(
Q8FJZ3
)
|
|
- Protein name
|
Rod shape-determining protein rodA.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=mrdB; OrderedLocusNames=c0725;
|
|
- Comments
|
SIMILARITY:Belongs to the ABC transporter family.
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
mrdB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Rod shape-determining protein rodA
|
|
PSI-BLAST
|
Rod shape-determining protein rodA
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
rod shape-determining membrane protein; sensitivity to radiation and drugs [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PHOSPHOPANTETHEINE
FTSW_RODA_SPOVE
|
|
MMBR References
|
Eur J Biochem. 1986;160:231-238
Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K-12.
Asoh, S., H. Matsuzawa, F. Ishino, J. L. Strominger, M. Matsuhashi, and T. Ohta.
|
J Bacteriol. 1983;154(1):10-6.
Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids.
Asoh S, Matsuzawa H, Matsuhashi M, Ohta T.
(
6300030
)
|
J Bacteriol. 1985;163(2):615-22.
Cell shape and division in Escherichia coli: experiments with shape and division mutants.
Begg KJ, Donachie WD.
(
3894330
)
|
J Bacteriol. 1989;171(1):558-60.
Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon.
Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T.
(
2644207
)
|
J Bacteriol. 1983;155(2):847-53.
Organization and subcloning of the dacA-rodA-pbpA cluster of cell shape genes in Escherichia coli.
Stoker NG, Broome-Smith JK, Edelman A, Spratt BG.
(
6348028
)
|
J Bacteriol. 1983;155(2):854-9.
Identification of the rodA gene product of Escherichia coli.
Stoker NG, Pratt JM, Spratt BG.
(
6348029
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTDNPNKKTF WDKVHLDPTM LLILLALLVY SALVIWSASG QDIGMMERKI GQIAMGLVIM VVMAQIPPRV
0071 YEGWAPYLYI ICIILLVAVD AFGAISKGAQ RWLDLGIVRF QPSEIAKIAV PLMVARFINR DVCPPSLKNT
0141 GIALVLIFMP TLLVAAQPDL GTSILVALSG LFVLFLSGLS WRLIGVAVVL VAAFIPILWF FLMHDYQRQR
0211 VMMLLDPESD PLGAGYHIIQ SKIAIGSGGL RGKGWLHGTQ SQLEFLPERH TDFIFAVLAE ELGLVGILIL
0281 LALYILLIMR GLWIAARAQT TFGRVMAGGL MLILFVYVFV NIGMVSGILP VVGVPLPLVS YGGSALIVLM
0351 AGFGIVMSIH THRKMLSKSV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cactgatgcg ccagatcctc gaccacatta
-070 tgctgggtga taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
0001 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgatct
0071 tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
0141 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt
0211 tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggtg
0281 ccatctctaa aggtgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa
0351 aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
0421 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa
0491 tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
0561 agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc
0631 gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg
0701 ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc
0771 cgaacgccat actgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
0841 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc
0911 gcgtcatggc tggcggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
0981 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg
1051 gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc
1121 gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg
1191 gtcagcaaca gacggtaagt gta
|
