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PEC Original Annotations
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Essentiality
|
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Class
|
non-essential
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|
References (PMID)
|
Carcinogenesis. 1993;14(2):303-5.
A method for selection of forward mutations in supF gene carried by shuttle-vector plasmids.
Ariza RR, Roldan-Arjona T, Hera C, Pueyo C.
(
8435871
)
|
|
|
Deletion
|
OCR29
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
araD
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Related strains
|
|
Strains Search
|
Search strains by gene name:
araD
Search strains by all related name:
araD b0061 ECK0062 JW0060
|
General information (Go to Linear View: )
|
|
Gene Name
|
araD
|
|
Alternative name
|
b0061,ECK0062,JW0060
|
|
Location, Length
|
65,855 - 66,550
(
-
)
;
1.42
min
;
696
(bp) ,
231
(aa)
|
|
Product
|
L-ribulose-5-phosphate 4-epimerase
|
|
Operon Name
|
araBAD
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
|
Gene Ontology
|
GO:0005975
;
carbohydrate metabolic process ( araD )
GO:0008270
;
zinc ion binding ( araD )
GO:0008742
;
L-ribulose-phosphate 4-epimerase activity ( araD )
GO:0016853
;
isomerase activity ( araD )
GO:0019568
;
arabinose catabolic process ( araD )
GO:0019572
;
L-arabinose catabolic process ( araD )
GO:0046872
;
metal ion binding ( araD )
|
|
PID
|
1786247
|
EC number
(KEGG Pathway)
|
5.1.3.4
|
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
ARAD_ECOLI
(
P08203
)
|
|
- Protein name
|
L-ribulose-5-phosphate 4-epimerase
|
|
- Synonyms
|
EC 5.1.3.4, Phosphoribulose isomerase
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|
- Gene name
|
Name=araD; OrderedLocusNames=b0061;
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- Comments
|
CATALYTIC ACTIVITY:L-ribulose 5-phosphate = D-xylulose 5-phosphate.
COFACTOR:Binds 1 zinc ion per subunit (Potential).
PATHWAY:L-arabinose catabolism; third step.
SIMILARITY:Belongs to the aldolase class II family. AraD/fucA subfamily.
|
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Entry name(Acc.no)
|
Q8FL90
(
Q8FL90
)
|
|
- Protein name
|
L-ribulose-5-phosphate 4-epimerase
|
|
- Synonyms
|
EC 5.1.3.4
|
|
- Gene name
|
Name=araD; OrderedLocusNames=c0073;
|
|
- Comments
|
SIMILARITY:Belongs to the ABC transporter family.
|
|
Entry name(Acc.no)
|
Q8XA08
(
Q8XA08
)
|
|
- Protein name
|
L-ribulose-5-phosphate 4-epimerase.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=araD; OrderedLocusNames=z0069;
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- Comments
|
SIMILARITY:Belongs to the ABC transporter family.
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Homology Analysis
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|
BLAST
|
|
Bacteria
|
|
GTOP
|
araD
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Crystal Structure Of L-Ribulose-5-Phosphate 4-Epimerase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
L-ribulose-5-phosphate 4-epimerase
|
|
PSI-BLAST
|
L-ribulose-5-phosphate 4-epimerase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
L-ribulose-5-phosphate 4-epimerase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
Gene. 1986;47(2-3):231-44.
The organization of the araBAD operon of Escherichia coli.
Lee N, Gielow W, Martin R, Hamilton E, Fowler A.
(
3549454
)
|
Nucleic Acids Res. 1990;18(22):6722
Nucleotide sequence of the araD gene of Escherichia coli K12 encoding the L-ribulose 5-phosphate 4-epimerase.
Mineno J, Fukui H, Ishino Y, Kato I, Shinagawa H.
(
2251150
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MLEDLKRQVL EANLALPKHN LVTLTWGNVS AVDRERGVFV IKPSGVDYSV MTADDMVVVS IETGEVVEGT
0071 KKPSSDTPTH RLLYQAFPSI GGIVHTHSRH ATIWAQAGQS IPATGTTHAD YFYGTIPCTR KMTDAEINGE
0141 YEWETGNVIV ETFEKQGIDA AQMPGVLVHS HGPFAWGKNA EDAVHNAIVL EEVAYMGIFC RQLAPQLPDM
0211 QQTLLDKHYL RKHGAKAYYG Q
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cattcaacgc ctgatgcgac gctggcgcgt
-070 cttatcaggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcacga aggagtcaac
0001 atgttagaag atctcaaacg ccaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc
0071 tcacatgggg caacgtcagc gccgttgatc gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga
0141 ttacagcgtc atgaccgctg acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg
0211 aaaaagccct cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
0281 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa ccggcaccac
0351 ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg acgcagaaat caacggcgaa
0421 tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg aaaaacaggg tatcgatgca gcgcaaatgc
0491 ccggcgttct ggtccattcc cacggcccgt ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc
0561 catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg
0631 cagcaaacgc tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
0701 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg gattttcagc
0771 gtggcgcagg caggttttat cttaac
|
