|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR29
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rluA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rluA
Search strains by all related name:
rluA b0058 ECK0059 JW0057 yabO
|
General information (Go to Linear View: )
|
|
Gene Name
|
rluA
|
|
Alternative name
|
b0058,ECK0059,JW0057,yabO
|
|
Location, Length
|
59,687 - 60,346
(
-
)
;
1.29
min
;
660
(bp) ,
219
(aa)
|
|
Product
|
dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM-dependent
|
|
Operon Name
|
hepA-rluA
|
|
Note
|
GO_process: GO:0009451 - RNA modification
|
|
Function
|
enzyme; rRNA modification; tRNA modification
|
|
Gene Ontology
|
GO:0001522
;
pseudouridine synthesis ( rluA )
GO:0003723
;
RNA binding ( rluA )
GO:0006364
;
rRNA processing ( rluA )
GO:0008033
;
tRNA processing ( rluA )
GO:0009982
;
pseudouridine synthase activity ( rluA )
GO:0016853
;
isomerase activity ( rluA )
|
|
PID
|
1786244
|
EC number
(KEGG Pathway)
|
4.2.1.70
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
rluA
(
EG12609
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
RLUA_ECOLI
(
P39219
;
Q83MG7
)
|
|
- Protein name
|
Ribosomal large subunit pseudouridine synthase A
|
|
- Synonyms
|
EC 4.2.1.70, Pseudouridylate synthase, Uracil hydrolyase
|
|
- Gene name
|
Name=rluA; OrderedLocusNames=b0058, SF0053, S0055;
|
|
- Comments
|
FUNCTION:Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs.
CATALYTIC ACTIVITY:Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O.
SIMILARITY:Belongs to the pseudouridine synthase rluA family.
CAUTION:Ref.3 sequence differs from that shown due to a frameshift in position 188.
|
|
Entry name(Acc.no)
|
RLUA_ECOL6
(
Q8FL93
)
|
|
- Protein name
|
Ribosomal large subunit pseudouridine synthase A
|
|
- Synonyms
|
EC 4.2.1.70, Pseudouridylate synthase, Uracil hydrolyase
|
|
- Gene name
|
Name=rluA; OrderedLocusNames=c0069;
|
|
- Comments
|
FUNCTION:Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs (By similarity).
CATALYTIC ACTIVITY:Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O.
SIMILARITY:Belongs to the pseudouridine synthase rluA family.
|
|
Entry name(Acc.no)
|
RLUA_ECO57
(
Q8XA10
)
|
|
- Protein name
|
Ribosomal large subunit pseudouridine synthase A
|
|
- Synonyms
|
EC 4.2.1.70, Pseudouridylate synthase, Uracil hydrolyase
|
|
- Gene name
|
Name=rluA; OrderedLocusNames=z0066, ECs0062;
|
|
- Comments
|
FUNCTION:Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs (By similarity).
CATALYTIC ACTIVITY:Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O.
SIMILARITY:Belongs to the pseudouridine synthase rluA family.
|
|
MMBR References
|
Nucleic Acids Res. 1996;24:3756-3762
Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes.
Gustafsson, C., R. Reid, P. J. Greene, and D. V. Santi.
|
J Biol Chem. 1988;263:2255-2263
Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I.
Kammen, H. O., C. C. Marvel, L. Hardy, and E. E. Penhoet.
|
RNA 1995;1:437-448
A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Psi 746 in 23S RNA is also specific for Psi 32 tRNAPhe.
Wrzesinski, J., K. Nurse, A. Bakin, B. G. Lane, and J. Ofengand.
|
| Sequences |
Amino acid
FASTA format
|
0001 MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV HRLDMATSGV
0071 IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP NRPKQKVCYE TGKPAQTEYE
0141 VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF YASPEARAMA PRLLLHAEML TITHPAYGNS
0211 MTFKAPADF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agagcaaccg tcagcaggta atggaaagcc
-070 tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa
0001 atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat gaccatatta
0071 tggtggtcaa caagccgagc ggtttgttgt cagtgccggg tcgtctggaa gagcacaaag acagcgtgat
0141 gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg
0211 attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa
0281 agcagtatgt ggcccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg
0351 cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa
0421 gtggtggagt atgcggcgga taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcaat
0491 tgcgtgtgca tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag
0561 agcgatggca ccacgtttgt tgctacatgc agagatgttg acgattaccc atccggcgta tggcaatagt
0631 atgacgttta aagcgccagc ggatttttaa cctttcctgt acgtttatac cctcatccta agggctgcct
0701 cttgtacata aatccccgag tccggggatt ttttgtttct gccaaactgc tttcagttgt
|
