MG1655
W3110
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Gene Report : apaH
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Mol Biol. 1990;212(2):319-29.
Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.
Leveque F, Blanchin-Roland S, Fayat G, Plateau P, Blanquet S. ( 2157025 )
     Deletion OCR29 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  apaH
Related strains
     Strains Search Search strains by gene name:  apaH   Search strains by all related name:  apaH b0049 cfcB ECK0050 JW0048

General information  (Go to Linear View:)
 Gene Name apaH  
 Alternative name b0049,cfcB,ECK0050,JW0048  
 Location, Length 50,380 - 51,222 (  -  ) ;   1.09 min ; 843 (bp) ,   280 (aa) Go to Linear View
 Product diadenosine tetraphosphatase  
 Operon Name lptD-surA-pdxA-rsmA-apaGH  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion  
 Function enzyme; Salvage of nucleosides and nucleotides  
 Gene Ontology GO:0008803 ; bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity ( apaH )
GO:0016787 ; hydrolase activity ( apaH )
 PID 1786234  
 EC number
  (KEGG Pathway)   		3.6.1.41  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) APAH_ECOLI ( P05637 ; P78039 )
    -  Protein name Bis(5'-nucleosyl)-tetraphosphatase, symmetrical  
    -  Synonyms EC 3.6.1.41, Diadenosine tetraphosphatase, Ap4A hydrolase, Diadenosine 5',5'''- P1,P4-tetraphosphate pyrophosphohydrolase  
    -  Gene name Name=apaH; OrderedLocusNames=b0049;  
    -  Comments
  • FUNCTION:Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.
  • CATALYTIC ACTIVITY:P(1),P(4)-bis(5'-adenosyl)tetraphosphate + H(2)O = 2 ADP.
  • ENZYME REGULATION:Co(2+) is a strong stimulator (100-fold increase in rate of hydrolysis). Mn(2+), Cd(2+), Ni(2+), Mg(2+) and Ca(2+) are weak stimulators; the two latter act synergistically with Co(2+).
  • SUBUNIT:Monomer.
  • SIMILARITY:Belongs to the Ap4A hydrolase family.  
    •   botton Entry name(Acc.no) APAH_ECOL6 ( Q8FL98 )
        -  Protein name Bis(5'-nucleosyl)-tetraphosphatase, symmetrical  
        -  Synonyms EC 3.6.1.41, Diadenosine tetraphosphatase, Ap4A hydrolase, Diadenosine 5',5'''- P1,P4-tetraphosphate pyrophosphohydrolase  
        -  Gene name Name=apaH; OrderedLocusNames=c0061;  
        -  Comments
  • FUNCTION:Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP (By similarity).
  • CATALYTIC ACTIVITY:P(1),P(4)-bis(5'-adenosyl)tetraphosphate + H(2)O = 2 ADP.
  • SUBUNIT:Monomer (By similarity).
  • SIMILARITY:Belongs to the Ap4A hydrolase family.  
    •   botton Entry name(Acc.no) APAH_ECO57 ( Q8XA15 )
        -  Protein name Bis(5'-nucleosyl)-tetraphosphatase, symmetrical  
        -  Synonyms EC 3.6.1.41, Diadenosine tetraphosphatase, Ap4A hydrolase, Diadenosine 5',5'''- P1,P4-tetraphosphate pyrophosphohydrolase  
        -  Gene name Name=apaH; OrderedLocusNames=z0058, ECs0054;  
        -  Comments
  • FUNCTION:Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP (By similarity).
  • CATALYTIC ACTIVITY:P(1),P(4)-bis(5'-adenosyl)tetraphosphate + H(2)O = 2 ADP.
  • SUBUNIT:Monomer (By similarity).
  • SIMILARITY:Belongs to the Ap4A hydrolase family.  

    •  Linear View (Whole Mode)
    View Location
       40.0  –  65.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP apaH (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of The Diadenosine Tetraphosphate Hydrolase From Shigella Flexneri 2a  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Bis  
             PSI-BLAST Bis
        nr     (database updated : 2007.02.20 )
             BLAST diadenosinetetraphosphatase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG0639T 
        EcoCyc apaH 

    MMBR References
    Proc Natl Acad Sci U S A. 1989;86:5010-5014
    An apaH mutation causes AppppA to accumulate and affects motility and catabolite repression in Escherichia coli.
    Farr, S. B., D. N. Arnosti, M. J. Chamberlin, and B. N. Ames.
    EMBO J. 1991;10:3897-3904
    AppppA binds to several proteins in Escherichia coli, including the heat shock and oxidative stress proteins DnaK, GroEL, E89, C45, and C40.
    Johnstone, D. B., and S. B. Farr.
    J Mol Biol. 1990;212(2):319-29.
    Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.
    Leveque F, Blanchin-Roland S, Fayat G, Plateau P, Blanquet S. ( 2157025 )
    J Bacteriol. 1985;164(1):63-9.
    Molecular cloning of the Escherichia coli gene for diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase.
    Mechulam Y, Fromant M, Mellot P, Plateau P, Blanchin-Roland S, Fayat G, Blanquet S. ( 2995325 )
    J Bacteriol. 1989;171(9):4767-77.
    Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12.
    Roa BB, Connolly DM, Winkler ME. ( 2670894 )
    Mol Gen Genet. 1986;205(3):515-22.
    The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA.
    Blanchin-Roland S, Blanquet S, Schmitter JM, Fayat G. ( 3031429 )

    Sequences
    Amino acid
    FASTA format 
    0001 MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR YVKSLGDSVR LVLGNHDLHL 
0071 LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQI DEEKKLVMAH AGITPQWDLQ TAKECARDVE 
0141 AVLSSDSYPF FLDAMYGDMP NNWSPELRGL GRLRFITNAF TRMRFCFPNG QLDMYSKESP EEAPAPLKPW 
0211 FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI YALDTGCCWG GTLTCLRWED KQYFVQPSNR HKDLGEAAAS
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             gcagggtcac tacgaaatga tcgatgaaaa 
-070 tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca cactcattca ttaaaagaat 
0001 atggcgacat accttattgg cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag 
0071 aatttacccc tgggaaagat accctctggc tgacgggcga tctggtcgcg cgcgggccgg gttcgctgga 
0141 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg 
0211 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagcgccgg 
0281 atgccgacga gctgcttaac tggctgcggc gccagcctct gctgcaaatc gacgaagaga aaaagctggt 
0351 gatggcccac gcagggatca cgccgcagtg ggatctgcag accgccaaag agtgcgcacg cgatgtagaa 
0421 gcggtgctat cgagtgactc ctatcccttc tttcttgatg ccatgtacgg cgatatgcca aataactggt 
0491 caccggaatt gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc gtttttgctt 
0561 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact gaaaccgtgg 
0631 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg ctggagggca 
0701 aaggtacgcc ggaaggtata tacgcgctgg ataccggctg ctgctggggt ggtacattaa cctgcctgcg 
0771 ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct 
0841 taaacacagc ctgatatagg aaggccggat aagacgcgac cggcgtcgca tccggcgcta gccgtaaatt 
0911 ctatacaaaa ttaccgccgc tccagaatct caa