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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Exp Biol. 1994;196:443-56.
Molecular physiology of the Na+/H+ antiporter in Escherichia coli.
Padan E, Schuldiner S.
(
7823039
)
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Deletion
|
OCR27
(D)
,
OCR27-8
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
nhaA
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Related strains
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Strains Search
|
Search strains by gene name:
nhaA
Search strains by all related name:
nhaA ant antA b0019 ECK0020 JW0018
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General information (Go to Linear View: )
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Gene Name
|
nhaA
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Alternative name
|
ant,antA,b0019,ECK0020,JW0018
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Location, Length
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17,489 - 18,655
(
+
)
;
0.38
min
;
1167
(bp) ,
388
(aa)
|
|
Product
|
sodium-proton antiporter
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Operon Name
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nhaAR
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Note
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Na+/H antiporter, pH dependent; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009268 - response to pH
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Function
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transport; Transport of small molecules: Cations
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Gene Ontology
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GO:0005886
;
plasma membrane ( nhaA )
GO:0006810
;
transport ( nhaA )
GO:0006811
;
ion transport ( nhaA )
GO:0006814
;
sodium ion transport ( nhaA )
GO:0006885
;
regulation of pH ( nhaA )
GO:0015297
;
antiporter activity ( nhaA )
GO:0016020
;
membrane ( nhaA )
GO:0016021
;
integral to membrane ( nhaA )
GO:0031402
;
sodium ion binding ( nhaA )
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PID
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1786201
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
nhaA
(
EG10652
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
NHAA_ECOLI
(
P13738
)
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- Protein name
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Na(+)/H(+) antiporter 1
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- Synonyms
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Sodium/proton antiporter 1
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- Gene name
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Name=nhaA; Synonyms=ant; OrderedLocusNames=b0019;
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- Comments
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FUNCTION:This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied. It probably acts in the regulation of internal pH at the alkaline pH range. It catalyzes the exchange of 2 H(+) per Na(+). Its activity is highly dependent on the pH.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
INDUCTION:Transcription stimulated by high Na(+) concentrations.
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Entry name(Acc.no)
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Q8FLC1
(
Q8FLC1
)
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- Protein name
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Na(+)/H(+) antiporter 1.
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- Synonyms
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- Gene name
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Name=nhaA; OrderedLocusNames=c0024;
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- Comments
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SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.
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Entry name(Acc.no)
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Q8XA63
(
Q8XA63
)
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- Protein name
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Na+/H antiporter, pH dependent.
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- Synonyms
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- Gene name
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Name=nhaA; OrderedLocusNames=z0018;
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- Comments
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SUBCELLULAR LOCATION:Integral membrane protein. Outer membrane (By similarity).
SIMILARITY:Belongs to the fimbrial export usher family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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nhaA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of The Na+H+ ANTIPORTER NHAA
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Na
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PSI-BLAST
|
Na
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nr
(database updated :
2007.02.20
)
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BLAST
|
pH-dependent sodium/proton antiporter [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
|
PKC_PHOSPHO_SITE
MYRISTYL
LEUCINE_ZIPPER
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MMBR References
|
J Bacteriol. 1996;178:6508-6517
Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns.
Dover, N., C. F. Higgins, O. Carmel, A. Rimon, E. Pinner, and E. Padan.
|
Proc Natl Acad Sci U S A. 1993;90:1212-1216
Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli.
Gerchman, Y., Y. Olami, A. Rimon, D. Taglicht, S. Schuldiner, and E. Padan.
|
Proc Natl Acad Sci U S A. 1987;84:2615-2619
Characterization of a Na+/H+ antiporter gene of Escherichia coli.
Goldberg, E. B., T. Arbel, J. Chen, R. Karpel, G. A. Mackie, and S. Schuldiner.
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Biol Pharm Bull. 1994;17(3):395-8.
Lithium toxicity and Na+(Li+)/H+ antiporter in Escherichia coli.
Inaba K, Kuroda T, Shimamoto T, Kayahara T, Tsuda M, Tsuchiya T.
(
8019504
)
|
J Biol Chem. 1991;266:21753-21759
Expression of a sodium proton antiporter (NhaA) in Escherichia coli is induced by Na+ and Li+ ions.
Karpel, R., T. Alon, G. Glaser, S. Schuldiner, and E. Padan.
|
J Biol Chem. 1988;263:10408-10414
Sequencing of the gene ant which affects the Na+/H+ antiporter activity in Escherichia coli.
Karpel, R., Y. Olami, D. Taglicht, S. Schuldiner, and E. Padan.
|
J Bacteriol. 1992;174:7743-7749
Escherichia coli is able to grow with negligible sodium ion extrusion activity at alkaline pH.
Ohyama, T., R. Imaizumi, K. Igarashi, and H. Kobayashi.
|
J Biol Chem. 1989;264:20297-20302
Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter systems(s).
Padan, E., N. Maisler, D. Taglicht, R. Karpel, and S. Schuldiner.
|
J Bioenerg Biomembr. 1993;25(6):647-69.
Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells.
Padan E, Schuldiner S.
(
8144493
)
|
J Biol Chem. 1992;267:10433-10438
NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR) regulates nhaA, the sodium proton antiporter gene in Escherichia coli.
Rahav-Manor, O., O. Carmel, R. Karpel, D. Taglicht, G. Glaser, S. Schuldiner, and E. Padan.
|
J Biol Chem. 1991;266:11289-11294
Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli.
Taglicht, D., E. Padan, and S. Schuldiner.
|
J Bacteriol. 1991;173(20):6553-7.
Characterization and mapping of a major Na+/H+ antiporter gene of Escherichia coli.
Thelen P, Tsuchiya T, Goldberg EB.
(
1655717
)
|
FEBS Lett. 1996;388(2-3):217-8.
The respiration-driven active sodium transport system in E. coli does not function with lithium.
Verkhovskaya ML, Verkhovsky MI, Wikstrom M.
(
8690090
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV GSLEINKNML LWINDALMAV
0071 FFLLVGLEVK RELMQGSLAS LRQAAFPVIA AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV
0141 LALLGSRVPL ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC GARRTGVYIL
0211 VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR LEHVLHPWVA YLILPLFAFA NAGVSLQGVT
0281 LDGLTSILPL GIIAGLLIGK PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF
0351 GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TAAAAAACGA ATTATTCCTA CACTATAATC
-070 TGATTTTAAC GATGATTCGT GCGGGGTAAA ATAGTAAAAA CGATCTATTC ACCTGAAAGA GAAATAAAAA
0001 gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc
0071 tggcgatgat tatggccaac agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca
0141 gctccgggtt ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct gatggcggta
0211 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc ttacgccagg
0281 ccgcatttcc agttatcgcc gctattggtg ggatgattgt gccggcatta ctctatctgg cttttaacta
0351 tgccgatccg attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcttttgc acttggtgta
0421 ctggcgctgt tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg
0491 atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc
0561 ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgcacgcc gcacgggcgt ctatattctt
0631 gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac tctggcgggg gtaattgtcg
0701 gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc
0771 gtgggtggcg tatctgattt tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggcgtcacg
0841 ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa ccgctgggga
0911 ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca
0981 aattatggtg gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
1051 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc tcttcggcgg
1121 taattggata cagctggtta cgcgttcgtt tgcgtccatc agtttgaCAG GACGGTTTAC CGGGGAGCCA
1191 TAAACGGCTC CCTTTTCATT GTTATCAGGG AGAGAAATGA GCATGTCTCA TATCAATTAC AACCACTTGT
1261 ATTACTT
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