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PEC Original Annotations
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|
Essentiality
|
|
Class
|
essential
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|
References (PMID)
|
J Bacteriol. 1992;174(23):7517-26.
Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli.
Yamanaka K, Ogura T, Niki H, Hiraga S.
(
1447125
)
|
|
|
Deletion
|
OCR33,34-1
(D)
,
OCR33,34-2
(D)
,
OCR33,35-4 (AK4-2 / BK5-2)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
pyrH
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
pyrH
Search strains by all related name:
pyrH b0171 ECK0170 JW0166 smbA umk
|
General information (Go to Linear View: )
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Gene Name
|
pyrH
|
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Alternative name
|
b0171,ECK0170,JW0166,smbA,umk
|
|
Location, Length
|
191,855 - 192,580
(
+
)
;
4.14
min
;
726
(bp) ,
241
(aa)
|
|
Product
|
uridylate kinase
|
|
Operon Name
|
pyrH
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
|
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Function
|
enzyme; Central intermediary metabolism: Nucleotide interconversions
|
|
Gene Ontology
|
GO:0005515
;
protein binding ( pyrH )
GO:0006221
;
pyrimidine nucleotide biosynthetic process ( pyrH )
GO:0008652
;
amino acid biosynthetic process ( pyrH )
GO:0009041
;
uridylate kinase activity ( pyrH )
GO:0016301
;
kinase activity ( pyrH )
GO:0016740
;
transferase activity ( pyrH )
|
|
PID
|
1786367
|
EC number
(KEGG Pathway)
|
2.7.4.-
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
pyrH
(
EG11539
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
PYRH_ECOLI
(
P29464
)
|
|
- Protein name
|
Uridylate kinase
|
|
- Synonyms
|
EC 2.7.4.-, UK, Uridine monophosphate kinase, UMP kinase
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- Gene name
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OrderedLocusNames=b0171, c0207, z0182, ECs0173, SF0161, S0164;
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- Comments
|
FUNCTION:Catalyzes the phosphorylation of UMP to UDP, with ATP as preferred donor.
CATALYTIC ACTIVITY:ATP + UMP = ADP + UDP.
ENZYME REGULATION:Allosterically activated by GTP and inhibited by UTP.
PATHWAY:Pyrimidine biosynthesis; conversion of UMP to CTP; first step.
SUBUNIT:Homohexamer.
SIMILARITY:Belongs to the UMP kinase family.
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
pyrH
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, The Structure Of E. Coli Ump Kinase In Complex With Ump
|
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SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Uridylate kinase (UK) (Uridine monophosphate kinase) (UMP kinase)
|
|
PSI-BLAST
|
Uridylate kinase (UK) (Uridine monophosphate kinase) (UMP kinase)
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
uridylate kinase [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
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PROSITE
|
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PROSITE
|
CK2_PHOSPHO_SITE
MYRISTYL
C5_MTASE_2
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MMBR References
|
Genetics (Life Sci. Adv.) 1992;11:59-65.
Cloning, nucleotide sequence and expression of the Escherichia coli K-12 pyrH gene encoding UMP kinase.
Smallshaw, J. E., and R. A. Kelln.
|
Mol Gen Genet. 1994;243(1):9-16.
Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli.
Yamanaka K, Ogura T, Koonin EV, Niki H, Hiraga S.
(
8190075
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MATNAKPVYK RILLKLSGEA LQGTEGFGID ASILDRMAQE IKELVELGIQ VGVVIGGGNL FRGAGLAKAG
0071 MNRVVGDHMG MLATVMNGLA MRDALHRAYV NARLMSAIPL NGVCDSYSWA EAISLLRNNR VVILSAGTGN
0141 PFFTTDSAAC LRGIEIEADV VLKATKVDGV FTADPAKDPT ATMYEQLTYS EVLEKELKVM DLAAFTLARD
0211 HKLPIRVFNM NKPGALRRVV MGEKEGTLIT E
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TTGTAAATTC AGCTAACCCT TGTGGGGCTG
-070 CGCTGAAAAG CGACGTACAA TGTCGCTAGT ATTAATTCAT TTCAATCGTT GACAGTCTCA GGAAAGAAAC
0001 atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
0071 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact
0141 gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
0211 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg
0281 cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta
0351 cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac
0421 ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
0491 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact
0561 gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
0631 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa
0701 aagaagggac tttaatcacg gaataaTTCC CGTGATGGAT AAATAAGGGT AAGATTCCGC GTAAGTATCG
0771 CGGGGGCGTA AGTCTGGTTA TAAGGCGTTA TTGTTGCAGG CAGTTTGGTC ACGGCC
|
