MG1655
W3110
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Gene Report : folK
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1992;174(18):5971-7.
Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.
Talarico TL, Ray PH, Dev IK, Merrill BM, Dallas WS. ( 1325970 )
     Deletion OCR32 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  folK
Related strains
     Strains Search Search strains by gene name:  folK   Search strains by all related name:  folK b0142 ECK0141 JW0138

General information  (Go to Linear View:)
 Gene Name folK  
 Alternative name b0142,ECK0141,JW0138  
 Location, Length 157,253 - 157,732 (  -  ) ;   3.39 min ; 480 (bp) ,   159 (aa) Go to Linear View
 Product 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase  
 Operon Name pcnB-folK  
 Note 7,8-dihydro-6-hydroxymethylpterin- pyrophosphokinase; GO_process: GO:0046656 - folic acid biosynthetic process  
 Function enzyme; Biosynthesis of cofactors, carriers: Folic acid  
 Gene Ontology GO:0003848 ; 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity ( folK )
GO:0009396 ; folic acid and derivative biosynthetic process ( folK )
GO:0016301 ; kinase activity ( folK )
GO:0016740 ; transferase activity ( folK )
GO:0046656 ; folic acid biosynthetic process ( folK )
 PID 1786335  
 EC number
  (KEGG Pathway)   		2.7.6.3  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene folK ( EG11374 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HPPK_ECOLI ( P26281 )
    -  Protein name 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase  
    -  Synonyms EC 2.7.6.3, 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, PPPK  
    -  Gene name Name=folK; OrderedLocusNames=b0142;  
    -  Comments
  • CATALYTIC ACTIVITY:ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + 2-amino-7,8-dihydro-4-hydroxy-6-(diphosphooxymethyl)pteridine.
  • PATHWAY:Dihydrofolate biosynthesis; first step.
  • SUBUNIT:Monomer.
  • MISCELLANEOUS:This enzyme is heat stable.
  • SIMILARITY:Belongs to the HPPK family.  
    •   botton Entry name(Acc.no) Q8FL26 ( Q8FL26 )
        -  Protein name 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase  
        -  Synonyms EC 2.7.6.3  
        -  Gene name Name=folK; OrderedLocusNames=c0175;  
        -  Comments
  • SIMILARITY:Belongs to the ABC transporter family.  
    •   botton Entry name(Acc.no) Q8X911 ( Q8X911 )
        -  Protein name 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.  
        -  Synonyms  
        -  Gene name Name=folK; OrderedLocusNames=z0153;  
        -  Comments
  • SUBCELLULAR LOCATION:Outer membrane (By similarity).  

    •  Linear View (Whole Mode)
    View Location
       145.0  –  170.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP folK (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain , 6-Hydroxymethyl-7,8-Dihydropterin Pyrophosphokinase  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase  
             PSI-BLAST 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
        nr     (database updated : 2007.02.20 )
             BLAST 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    HPPK     

    Other Cross-Reference
        COG COG0801H 
        EcoCyc folK 

    MMBR References
    J Bacteriol. 1992;174:5971-5977
    Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.
    Talarico, T. L., P. H. Ray, I. K. Dev, B. M. Merrill, and W. S. Dallas.

    Sequences
    Amino acid
    FASTA format 
    0001 MTVAYIAIGS NLASPLEQVN AALKALGDIP ESHILTVSSF YRTPPLGPQD QPDYLNAAVA LETSLAPEEL 
0071 LNHTQRIELQ QGRVRKAERW GPRTLDLDIM LFGNEVINTE RLTVPHYDMK NRGFMLWPLF EIAPELVFPD 
0141 GEMLRQILHT RAFDKLNKW
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             cagaccaaaa agggatgctc aacgagctgg 
-070 atgaagaacc gtcaccgcgt cgtcgtactc gtcgtccacg caaacgcgca ccacgtcgtg agggtaccgc 
0001 atgacagtgg cgtatattgc cataggcagc aatctggcct ctccgctgga gcaggtcaat gctgccctga 
0071 aagcattagg cgatatccct gaaagccaca ttcttaccgt ttcttcgttt taccgcaccc caccgctggg 
0141 gccgcaagat caacccgatt acttaaacgc agccgtggcg ctggaaacct ctcttgcacc tgaagagcta 
0211 ctcaatcaca cacagcgtat tgaattgcag caaggtcgcg tccgcaaagc tgaacgctgg ggaccacgca 
0281 cgctggatct cgacatcatg ctgtttggta atgaagtgat aaatactgaa cgcctgaccg ttccgcacta 
0351 cgatatgaag aatcgtggat ttatgctgtg gccgctgttt gaaatcgcgc cggagttggt gtttcctgat 
0421 ggggagatgt tgcgtcaaat cttacataca agagcatttg acaaattaaa caaatggtaa tttatttata 
0491 ttgcgaatat aaatattttc tgaaaatcat atctcatctc tttccttaat tacgatttta gtttaaacat 
0561 ctctttgcgg aaataatata