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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCR32
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
cueO
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Related strains
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Strains Search
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Search strains by gene name:
cueO
Search strains by all related name:
cueO b0123 cuiD ECK0122 JW0119 o516 yacK
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General information (Go to Linear View: )
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Gene Name
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cueO
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Alternative name
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b0123,cuiD,ECK0122,JW0119,o516,yacK
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Location, Length
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137,083 - 138,633
(
+
)
;
2.95
min
;
1551
(bp) ,
516
(aa)
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Product
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multicopper oxidase (laccase)
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Operon Name
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cueO
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Note
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Function
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Gene Ontology
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GO:0005507
;
copper ion binding ( cueO )
GO:0016491
;
oxidoreductase activity ( cueO )
GO:0042597
;
periplasmic space ( cueO )
GO:0046872
;
metal ion binding ( cueO )
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PID
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1786314
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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cueO
(
EG12318
)
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Number of removed
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28 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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CUEO_ECOLI
(
P36649
;
P75655
)
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- Protein name
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Blue copper oxidase cueO precursor
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- Synonyms
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Copper efflux oxidase
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- Gene name
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Name=cueO; OrderedLocusNames=b0123;
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- Comments
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FUNCTION:Probably involved in periplasmic detoxification of copper by oxidizing Cu(I) to Cu(II) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds and the prevention of oxidative damage in the periplasm.
COFACTOR:This protein belongs to the multicopper oxidases which contain three distinct Cu centers known as type 1 or blue, type 2 or normal, and type 3 or coupled binuclear.
SUBUNIT:Monomer (Probable).
SUBCELLULAR LOCATION:Periplasmic. It is exported via the Tat pathway.
INDUCTION:By cueR, at increased levels of cytoplasmic cuprous ions.
DOMAIN:The methionine-rich domain could provide binding sites for exogenous copper ions. This methionine-rich region is probably important for copper tolerance in bacteria.
MISCELLANEOUS:This protein is sensitive to oxygen deprivation. It probably plays a significant role in copper efflux under aerobic conditions.
SIMILARITY:Belongs to the multicopper oxidase family.
SIMILARITY:Contains 3 plastocyanin-like domains.
CAUTION:Ref.1 sequence differs from that shown due to a frameshift in position 464.
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Entry name(Acc.no)
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Q8CWE2
(
Q8CWE2
)
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- Protein name
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Blue copper oxidase cueO.
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- Synonyms
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- Gene name
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Name=yacK; OrderedLocusNames=c0152;
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- Comments
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SUBCELLULAR LOCATION:Integral membrane protein. Outer membrane (By similarity).
SIMILARITY:Belongs to the fimbrial export usher family.
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Entry name(Acc.no)
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CUEO_ECO57
(
Q8X947
)
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- Protein name
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Blue copper oxidase cueO precursor
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- Synonyms
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Copper efflux oxidase
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- Gene name
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Name=cueO; OrderedLocusNames=z0133, ECs0127;
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- Comments
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FUNCTION:Probably involved in periplasmic detoxification of copper by oxidizing Cu(I) to Cu(II) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds and the prevention of oxidative damage in the periplasm (By similarity).
COFACTOR:This protein belongs to the multicopper oxidases which contain three distinct Cu centers known as type 1 or blue, type 2 or normal, and type 3 or coupled binuclear (By similarity).
SUBUNIT:Monomer (Probable).
SUBCELLULAR LOCATION:Periplasmic. It is exported via the Tat pathway (By similarity).
INDUCTION:By cueR, at increased levels of cytoplasmic cuprous ions (Probable).
DOMAIN:The methionine-rich domain could provide binding sites for exogenous copper ions. This methionine-rich region is probably important for copper tolerance in bacteria.
SIMILARITY:Belongs to the multicopper oxidase family.
SIMILARITY:Contains 3 plastocyanin-like domains.
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| Sequences |
Amino acid
FASTA format
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0001 MQRRDFLKYS VALGVASALP LWSRAVFAAE RPTLPIPDLL TTDARNRIQL TIGAGQSTFG GKTATTWGYN
0071 GNLLGPAVKL QRGKAVTVDI YNQLTEETTL HWHGLEVPGE VDGGPQGIIP PGGKRSVTLN VDQPAATCWF
0141 HPHQHGKTGR QVAMGLAGLV VIEDDEILKL MLPKQWGIDD VPVIVQDKKF SADGQIDYQL DVMTAAVGWF
0211 GDTLLTNGAI YPQHAAPRGW LRLRLLNGCN ARSLNFATSD NRPLYVIASD GGLLPEPVKV SELPVLMGER
0281 FEVLVEVNDN KPFDLVTLPV SQMGMAIAPF DKPHPVMRIQ PIAISASGAL PDTLSSLPAL PSLEGLTVRK
0351 LQLSMDPMLD MMGMQMLMEK YGDQAMAGMD HSQMMGHMGH GNMNHMNHGG KFDFHHANKI NGQAFDMNKP
0421 MFAAAKGQYE RWVISGVGDM MLHPFHIHGT QFRILSENGK PPAAHRAGWK DTVKVEGNVS EVLVKFNHDA
0491 PKEHAYMAHC HLLEHEDTGM MLGFTV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 TGTCGATTAA ATTGTGTCTG CGGCTTGACC
-070 TTCCCGTAAG GGGAAGGACT ATGCTCAACG TTTGATTTTG TTTCGCCTGC TTAAGAATAA GGAAATAACT
0001 atgcaacgtc gtgatttctt aaaatattcc gtcgcgctgg gtgtggcttc ggctttgccg ctgtggagcc
0071 gcgcagtatt tgcggcagaa cgcccaacgt taccgatccc tgatttgctc acgaccgatg cccgtaatcg
0141 cattcagtta actattggcg caggccagtc cacctttggc gggaaaactg caactacctg gggctataac
0211 ggcaatctgc tggggccggc ggtgaaatta cagcgcggca aagcggtaac ggttgatatc tacaaccaac
0281 tgacggaaga gacaacgttg cactggcacg ggctggaagt accgggtgaa gtcgacggcg gcccgcaggg
0351 aattattccg ccaggtggca agcgctcggt gacgttgaac gttgatcaac ctgccgctac ctgctggttc
0421 catccgcatc agcacggcaa aaccgggcga caggtggcga tggggctggc tgggctggtg gtgattgaag
0491 atgacgagat cctgaaatta atgctgccaa aacagtgggg tatcgatgat gttccggtga tcgttcagga
0561 taagaaattt agcgccgacg ggcagattga ttatcaactg gatgtgatga ccgccgccgt gggctggttt
0631 ggcgatacgt tgctgaccaa cggtgcaatc tacccgcaac acgctgcccc gcgtggttgg ctgcgcctgc
0701 gtttgctcaa tggctgtaat gcccgttcgc tcaatttcgc caccagcgac aatcgcccgc tgtatgtgat
0771 tgccagcgac ggtggtctgc tacctgaacc agtgaaggtg agcgaactgc cggtgctgat gggcgagcgt
0841 tttgaagtgc tggtggaggt taacgataac aaaccctttg acctggtgac gctgccggtc agccagatgg
0911 ggatggcgat tgcgccgttt gataagcctc atccggtaat gcggattcag ccgattgcta ttagtgcctc
0981 cggtgctttg ccagacacat taagtagcct gcctgcgtta ccttcgctgg aagggctgac ggtacgcaag
1051 ctgcaactct ctatggaccc gatgctcgat atgatgggga tgcagatgct aatggagaaa tatggcgatc
1121 aggcgatggc cgggatggat cacagccaga tgatgggcca tatggggcac ggcaatatga atcatatgaa
1191 ccacggcggg aagttcgatt tccaccatgc caacaaaatc aacggtcagg cgtttgatat gaacaagccg
1261 atgtttgcgg cggcgaaagg gcaatacgaa cgttgggtta tctctggcgt gggcgacatg atgctgcatc
1331 cgttccatat ccacggcacg cagttccgta tcttgtcaga aaatggcaaa ccgccagcgg ctcatcgcgc
1401 gggctggaaa gataccgtta aggtagaagg taatgtcagc gaagtgctgg tgaagtttaa tcacgatgca
1471 ccgaaagaac atgcttatat ggcgcactgc catctgctgg agcatgaaga tacggggatg atgttagggt
1541 ttacggtata aAAAGACCGT ATTCGGAAAT ATGCCCGGAT CTCTCCGGGC ATTAAACTGA TGACAAACGC
1611 AAATCTGCCT GATGCGCTAC GCTTATCAGG CCTACGCTAG C
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