|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Gene. 1993;126(1):115-7.
Deletion mutations in the speED operon: spermidine is not essential for the growth of Escherichia coli.
Xie QW, Tabor CW, Tabor H.
(
8472951
)
|
|
|
Deletion
|
OCR32
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
speE
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
speE
Search strains by all related name:
speE b0121 ECK0120 JW0117
|
General information (Go to Linear View: )
|
|
Gene Name
|
speE
|
|
Alternative name
|
b0121,ECK0120,JW0117
|
|
Location, Length
|
135,598 - 136,464
(
-
)
;
2.92
min
;
867
(bp) ,
288
(aa)
|
|
Product
|
spermidine synthase (putrescine aminopropyltransferase)
|
|
Operon Name
|
yacC-speED
|
|
Note
|
spermidine synthase = putrescine aminopropyltransferase; GO_process: GO:0006596 - polyamine biosynthetic process
|
|
Function
|
enzyme; Central intermediary metabolism: Polyamine biosynthesis
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( speE )
GO:0004766
;
spermidine synthase activity ( speE )
GO:0006596
;
polyamine biosynthetic process ( speE )
GO:0008295
;
spermidine biosynthetic process ( speE )
GO:0016740
;
transferase activity ( speE )
|
|
PID
|
1786312
|
EC number
(KEGG Pathway)
|
2.5.1.16
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
speE
(
EG10963
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
SPEE_ECOLI
(
P09158
)
|
|
- Protein name
|
Spermidine synthase
|
|
- Synonyms
|
EC 2.5.1.16, Putrescine aminopropyltransferase, SPDSY
|
|
- Gene name
|
Name=speE; OrderedLocusNames=b0121;
|
|
- Comments
|
CATALYTIC ACTIVITY:S-adenosylmethioninamine + putrescine = 5'-methylthioadenosine + spermidine.
ENZYME REGULATION:The activity is thought to be regulated mainly by the availability of decarboxylated S-adenosylmethionine.
PATHWAY:Spermidine biosynthesis from arginine; third (last) step.
SUBUNIT:Homodimer.
MISCELLANEOUS:Optimal pH is 10.4.
SIMILARITY:Belongs to the spermidine/spermine synthase family.
|
|
Entry name(Acc.no)
|
SPEE_ECOL6
(
Q8X951
)
|
|
- Protein name
|
Spermidine synthase
|
|
- Synonyms
|
EC 2.5.1.16, Putrescine aminopropyltransferase, SPDSY
|
|
- Gene name
|
Name=speE; OrderedLocusNames=c0150, z0131, ECs0125;
|
|
- Comments
|
CATALYTIC ACTIVITY:S-adenosylmethioninamine + putrescine = 5'-methylthioadenosine + spermidine.
PATHWAY:Spermidine biosynthesis from arginine; third (last) step.
SIMILARITY:Belongs to the spermidine/spermine synthase family.
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
speE
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Human Spermidine Synthase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Spermidine synthase
|
|
PSI-BLAST
|
Spermidine synthase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
spermidine synthase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
SPERMIDINE_SYNTHASE
|
|
MMBR References
|
J Biol Chem. 1987;262:16037-16040
The speEspeD operon of Escherichia coli. Formation and processing of a proenzyme form of S adenosylmethionine decarboxylase.
Tabor, C. W., and H. Tabor.
|
Proc Natl Acad Sci U S A. 1986;83:6040-6044
Spermidine synthase of Escherichia coli: localization of the speE gene.
Tabor, C. W., H. Tabor, and Q.-W. Xie.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD GVVQTTERDE FIYHEMMTHV
0071 PLLAHGHAKH VLIIGGGDGA MLREVTRHKN VESITMVEID AGVVSFCRQY LPNHNAGSYD DPRFKLVIDD
0141 GVNFVNQTSQ TFDVIISDCT DPIGPGESLF TSAFYEGCKR CLNPGGIFVA QNGVCFLQQE EAIDSHRKLS
0211 HYFSDVGFYQ AAIPTYYGGI MTFAWATDND ALRHLSTEII QARFLASGLK CRYYNPAIHT AAFALPQYLQ
0281 DALASQPS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gatacctttt tgtagaaata ttgaccgtgc
-070 atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt aacaaaggag gtatcaaccc
0001 atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtagataacg
0071 ttctgtatca tgaaaagacc gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat
0141 ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
0211 ccgctactgg cccatggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
0281 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg tatcgttctg
0351 ccgtcagtat ctacccaacc ataacgccgg tagctacgac gatccgcgct ttaagctggt gatcgacgat
0421 ggcgtcaatt tcgttaatca aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg
0491 gtcccggcga aagccttttc acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat
0561 cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
0631 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
0701 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt ttctcgcctc
0771 tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg ccttacctca gtatctgcaa
0841 gacgcactgg cttcacagcc gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta
0911 ataatctgac caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactg
|
