|
PEC Original Annotations
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|
Essentiality
|
|
Class
|
non-essential
|
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References (PMID)
|
|
|
Deletion
|
DE(nadC-aceF)72
,
OCR31
(D)
,
OCR31-2
(D)
,
OCR31-7
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
ampE
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ampE
Search strains by all related name:
ampE b0111 ECK0110 JW0107
|
General information (Go to Linear View: )
|
|
Gene Name
|
ampE
|
|
Alternative name
|
b0111,ECK0110,JW0107
|
|
Location, Length
|
119,281 - 120,135
(
+
)
;
2.57
min
;
855
(bp) ,
284
(aa)
|
|
Product
|
putative inner membrane protein
|
|
Operon Name
|
ampDE
|
|
Note
|
regulates ampC; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0042493 - response to drug
|
|
Function
|
regulator; Drug/analog sensitivity
|
|
Gene Ontology
|
GO:0005886
;
plasma membrane ( ampE )
GO:0016020
;
membrane ( ampE )
GO:0016021
;
integral to membrane ( ampE )
|
|
PID
|
1786301
|
EC number
(KEGG Pathway)
|
|
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SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
AMPE_ECOLI
(
P13017
)
|
|
- Protein name
|
AmpE protein.
|
|
- Synonyms
|
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- Gene name
|
Name=ampE; OrderedLocusNames=b0111, SF0108, S0110;
|
|
- Comments
|
FUNCTION:Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
INDUCTION:The level of ampE affects the basal level of beta-lactamase expression in the absence of ampD and in the presence of the ampR transcriptional regulator.
|
|
Entry name(Acc.no)
|
Q8FL50
(
Q8FL50
)
|
|
- Protein name
|
AmpE protein.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=ampE; OrderedLocusNames=c0130;
|
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- Comments
|
SIMILARITY:Belongs to the ABC transporter family.
|
|
Entry name(Acc.no)
|
Q8X970
(
Q8X970
)
|
|
- Protein name
|
Regulates ampC.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=ampE; OrderedLocusNames=z0121;
|
|
- Comments
|
SIMILARITY:Belongs to the 2-oxoacid dehydrogenase family.
SIMILARITY:Contains 3 lipoyl-binding domains.
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
ampE
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Protein ampE
|
|
PSI-BLAST
|
Protein ampE
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
predicted inner membrane protein [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
LEUCINE_ZIPPER
|
|
MMBR References
|
Mol Microbiol. 1989;3(8):1121-30.
Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer.
Honore N, Nicolas MH, Cole ST.
(
2607970
)
|
Mol Microbiol. 1989;3(8):1091-102.
Signalling proteins in enterobacterial AmpC beta-lactamase regulation.
Lindquist S, Galleni M, Lindberg F, Normark S.
(
2691840
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTLFTTLLVL IFERLFKLGE HWQLDHRLEA FFRRVKHFSL GRTLGMTIIA MGVTFLLLRA LQGVLFNVPT
0071 LLVWLLIGLL CIGAGKVRLH YHAYLTAASR NDSHARATMA GELTMIHGVP AGCDEREYLR ELQNALLWIN
0141 FRFYLAPLFW LIVGGTWGPV TLMGYAFLRA WQYWLARYQT PHHRLQSGID AVLHVLDWVP VRLAGVVYAL
0211 IGHGEKALPA WFASLGDFHT SQYQVLTRLA QFSLAREPHV DKVETPKAAV SMAKKTSFVV VVVIALLTIY
0281 GALV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACATGACGGG CCATTGTGAT ATTGCGCCGG
-070 ATCGGAAAAC CGATCCCGGT CCTGCATTTG ATTGGGCACG GTTTCGTGTG CTGGTCAGCA AGGAGACAAC
0001 atgacgctat ttacaacctt actggtgtta attttcgagc gcctgtttaa gttgggcgag cactggcagc
0071 ttgatcatcg tcttgaagcg ttctttcggc gggtgaaaca tttttctctc gggcgcacgt taggcatgac
0141 cattattgcg atgggcgtga cttttttact gttacgcgca ttgcagggag tattgttcaa cgttcccacg
0211 ctactggtgt ggctgctgat tggtttgctg tgtattggcg caggtaaagt tcgtcttcat tatcatgctt
0281 atctgacagc tgcttcacgt aatgatagcc atgcccgtgc cacgatggct ggcgaactca ccatgattca
0351 cggcgtcccg gcaggctgcg acgaacgtga gtatttgcgt gagctgcaaa atgcattgct gtggattaac
0421 tttcgttttt atcttgcacc gctgttctgg ctgattgtgg ggggaacctg gggacccgtt acgctgatgg
0491 ggtatgcgtt tttgcgtgca tggcaatact ggctggcgcg atatcagacg ccgcatcatc gtttacagtc
0561 cggcattgat gccgtgcttc atgtactgga ttgggtgccg gttcgtcttg cgggtgtggt atatgccttg
0631 atcggtcatg gtgagaaagc gttaccggcc tggtttgctt cgctgggtga tttccatact tcgcagtatc
0701 aggtgttaac gcgtctggcg cagttctctc tggcgcgtga accgcatgtc gataaggtgg agacgccgaa
0771 ggcagcggtt tcaatggcga agaaaacctc gttcgtggtc gtggtggtga ttgcactact gacgatttac
0841 ggggcgttgg tgtaaAGATT ATTGCCCTCA CCCTGTACGG GTGAGGGCGT AGAGAGATTA ATGCGCTTTT
0911 ACGGCTTTGG CGGTTTTCTC TTTAAACAGA TAGCCGATAC CTAAC
|
