GFP expression in skin, Figure 2. Time lapse imaging of single MTs. Serial images of a skin cell in the SAGFF73A;UAS:Gtuba2 larvae at 108 hpf. The GFP signal is shown in black. The MT that was present just before shrinking is indicated by the arrowhead. The shrinking end of the MT is indicated by the white arrow. The shrinking end is not evident in the next frame. The growing end of the other MT is indicated by the black arrow. The growth rate of this MT is in a range of 13.0-25.9 ?m/min from t=128.2 to 139.8, which is slightly faster than that observed in mammalian LLCPK-1 cells (Rusan et al., 2001). The oval-shaped GFP-negative region (N) is the nucleus. The position of the putative MT-organizing center is indicated by the double arrowhead. The numbers in white indicate the time points in seconds. The bar indicates 2 ?m. at day 4.
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GFP expression in brain/head, (hindbrain), Figure 3. Time lapse imaging of mitosis in the developing hindbrain. (A) The dorsal view of the developing cerebellar region in the hspGGFF27A:UAS:Gtuba2 embryo at 54 hpf. The anterior is to the left. White dotted lines demarcate the developing cerebellum. The yellow dotted line indicates the midline. The division of the progenitor cell in the yellow box is monitored by time lapse imaging. A and b indicate the apical and basal side of the cerebellum, respectively. The bar indicates 10 ?m. (B) Time lapse imaging of a progenitor cell in the yellow box in A. White arrowheads indicate the position of the centrosomes before separation. Yellow arrowheads indicate the separated centrosomes. The double arrowhead (t = 46) indicates the position of the cleavage furrow. The positions of the centrosomes were unclear at t = 26. The numbers in white indicate the time points in minutes. The bar indicates 5?m. at day 4.
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