Strain Name: Tg(EF1α:mAG-hGem(1/60)) / Allele: rw0412a

Allele rw0412a
Strain Name Tg(EF1α:mAG-hGem(1/60))
Type Transgenics
Genotype
Affected Gene
Origin and Depositor RIKEN CBS (Atsushi Miyawaki)
Link to ZFIN
Information
Fucci is a fluorescent cell cycle indicator that harnesses the ubiquitination oscillators that control cell cycle transitions (Sakaue-Sawano et al., 2008 Cell). The original Fucci probe was generated by fusing mAG and mKO2 to ubiquitination domains of human Geminin and Cdt1, respectively. We describe here the generation of a Fucci derivative using zebrafish homologs of Cdt1 and Geminin, which provides sweeping views of cell proliferation in whole fish embryos. We also described that human geminin-Fucci can work on zebrafish.

Tg(EF1α:mAG-hGem(1/60))rw0412a shows green fluorescence in S/G2/M phases nucleocytoplasm in whole embryo.  
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The Conditions to Distribute Strains
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE in compliance with the following terms and conditions requested by the DEPOSITOR and which are specified on NZC homepage:

The BIOLOGICAL RESOURCE created by Dr. Shin-ichi Higashijima, the DEPOSITOR's collaborator, is covered by the DEPOSITOR's patented Fucci Technology that contains fluorescent proteins: mKO2 and mAG created by the DEPOSITOR and its collaborator Amalgaam Co., Ltd. Also, the BIOLOGICAL RESOURCE contains pT2KXIGΔ in vector (patented) provided by Dr. Koichi Kawakami, National Institute of Genetics.

The RECIPIENT shall acknowledge the following: The DEPOSITOR obtained the permission of its collaborators to deposit the BIOLOGICAL RESOURCE on the condition that the RECIPIENT agrees to use the BIOLOGICAL RESOURCE for non-profit research purposes and in compliance with the terms and conditions in this MATERIAL TRANSFER AGREEMENT.

The RECIPIENT agrees to acknowledge the DEPOSITOR and Dr. Shin-ichi Higashijima in any publications, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.

The RECIPIENT agrees to cite the following manuscript in publishing the research results related to the BIOLOGICAL RESOURCE.  
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Reference

Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo. Proc. Natl. Acad. Sci. U.S.A. 2009 ; 106(49) : 20812-20817 .

 
Facility RIKEN Center for Brain Science