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Wheat Information Service
Number 82: 29-30 (1996)
Research Information

II. Research Information

Transfer of stripe rust resistance to Unnath Sonalika and Unnath Kalyansona

R. N. Brahma and M Sivasamy

Indian Agricultural Research Institute, Regional Station, Wellington-643231, Tamil Nadu, India


Unnath Sonalika and Unnath Kalyansona, improved varieties of Sonalika and Kalyansona respectively (Kochumadhaven et al. 1988), carrying linked resistance genes Sr24 + Lr24,
were initially susceptible only to stripe rust. However, recently these varieties also became susceptible to stem rust. An attempt was made to transfer unidentified stripe rust resistance from the two hexaploid wheat lines in the background of Unnath Sonalika and Unnath Kalyansona.

Crosses of Unnath Sonalika and Unnath Kalyansona with the two wheat lines,. i.e., CPAN 3057 and CPAN 3063, were made at Indian Agricultural Research Institute, Regional Station, Wellington, The Nilgiris, South India. The location is a 'hot spot' for all wheat diseases, where all the three wheat rusts occur in epiphytotic form throughout the year. Resistant hybrids were backcrossed to recurrent parents and lines showing resistance to stem, leaf and stripe rusts were constituted after fifth successive backcrosses. Screening for rust resistance was made under natural epiphytotic conditions.

Crosses of Unnath Sonalika and Unnath Kalyansona with the two wheat lines were successful. The lines constituted from the respective crosses between Unnath Sonalika and the two wheat lines were completely free to stripe rust, while the lines from the crosses between Unnath Kalyansona and the two wheat lines showed low incidence of stripe rust (TMR) (
Table 1). These two wheat lines thus appeared to he good contributor for stripe rust resistance, though these failed to provide immunity to stripe rust in the latter case indicating that these lines are not virtually immune to stripe rust. It appears that a heavy inoculum load on the lines also leads to the breakdown of genetic resistance.

Interestingly, the new lines also exhibited good resistance to stem rust (TR), while Unnath Sonalika and Unnath Kalyansona were susceptible to stem rust (
Table 1). Unnath Sonalika and Unnath Kalyansona and their derivatives were immune to leaf rust indicating the effectiveness of leaf rust resistance gene Lr24. The reason for breakdown of effective resistance gene Sr24 in Unnath Sonalika and Unnath Kalyansona is not exactly known. Crossing-over between the two resistance genes can be ruled out, since these two genes are tightly linked to each other (McIntosh 1988). Occurrence of stem rust on a new resistance line could be due to previously unknown or new race with matching pathogenecity. However, under the same experimental conditions at Wellington, the original Australian line TR-380 14* 7/3 Ag 14 from which the linked resistance genes Sr24 + Lr24 were transferred to Unnath Sonalika and Unnath Kalyansona, was free to stem rust. The stem rust race involved in the attack of Unnath Sonalika and Unnath Kalyansona was also not race 40-1 which is known to knock down the resistance gene Sr24, but it was only 40A, a common virulence of the Nilgiri hills. However, the Australian line was quite effective against the race 40A.

Thus, good resistance observed against stem rust in the new wheat lines possibly was due to combined effect of resistance gene Sr24
and the additional unidentified gene(s) transferred from the two wheat lines which were resistant to stem rust (Table 1). It has been reported that rust resistance provided by single gene is not effective and durable resistance is provided only by a few gene combination (McIntosh 1988; Roelfs 1988; Roelfs et al. 1992). Since the new lines were simultaneously resistant to stem and stripe rust, it is possible that the resistance genes for stem and stripe rust derived from the donor parents are linked to each other.

Reference

Kochumadhavan K, Tomar SMS and Nambisan PNN (1988) Transfer of rust resistance genes into commercial cultivars of wheat. Ann Wheat News Letter 34: 54-55.

McIntosh RA (1988) The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. In: Breeding strategies for resistance to the rusts of wheat. Ed:NW Simonds and S. Rajaram. CIMMYT Report: 1-9.

Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Breeding strategies for resistance to the rusts of wheat. Ed:NW Simonds and S. Rajaram. CIMMYT Report: 10-22.

Roelfs AP, Singh RP and Saari EE (1992) Rust disease of wheat: Concepts and methods of disease management. CIMMYT Report 81.

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