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Wheat Information Service
Number 77: 23-24 (1993)


Characterization of hexaploid derivatives for Ne1 and Ne2 necrotic genes of wheat

Sanjiv Gupta and A. K. Gupta*

Biotechnology Centre, Punjab Agricultural University, Ludhiana- 141004, Punjab, India


Summary

Three hexaploid derivatives D 525-2 (from CPAN 6051), D 3237-5 (from DWL 5023) and D 3542-6 (from CPAN 6073) identified meiotically from the F2 population of the crosses between Triticum
durum and T. aestivum were characterized for hybrid necrotic genes Ne1 and Ne2. These derivatives in crosses with T. aestivum cvs. Spica (carrier of Ne1) and Kalyan Sona (carrier of Ne2) were evaluated in the field for necrosis. Derivative D 525-2 carries Ne2 and Ne1 is present in D 3237-5 and D 3542-6. Being resistant to leaf rust of wheat the derivatives can be utilized in the breeding programmes.


Introduction

Transfer of useful traits in wheat through intervarietal crosses is mainly restricted by the action of dominant complementary hybrid necrotic genes, Ne1 and Ne2 (Hermsen 1963). Ne1 is present in most of the durum wheats (Nishikawa 1967; Zeven 1981) and Ne2 in 70% of aestivum wheats (Mclntosh 1983). Classification of cultivars carrying these genes is very essential for the proper analyses and utilization of the breeding material in wheat improvement. Keeping this in view, the present investigations were carried out with the characterization of necrotic genes in three hexaploid derivatives.


Materials and methods

The derivatives were identified meiotically from the F2 population of the crosses between Triticum
durum cvs. CPAN 6051, DWL 5023 and CPAN 6073 and a Triticum aestivum cv. Agra Local. These derivatives were designated as D 525- 2 (from CPAN 6051), D 3237-5 (from DWL 5023) and D 3542-6 (from CPAN 6073). Each derivative was crossed with T. aestivum cvs. Spica (carrier of Ne1) and Kalyan Sona (carrier of Ne2) to detect necrotic genes. F1's were raised in 100 cms long rows spaced 50 cms apart in an open experimental area. The plants were observed throughout the wheat season at a weekly intervals to study the necrosis.


*Director, C. S. I. R. Complex, Palampur- 176061, Himachal Pradesh, India.

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