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Wheat Information Service
Number 76:49 (1993)


Three waxy (Wx) proteins in common wheat (Triticum aestivum L.)

T. Nakamura

Tohoku National Agricultural Experiment Station, Morioka, Iwate, Japan


Nullisomic analysis of waxy (Wx) protein of Triticum aestivum L. cv. "Chinese Spring" using two dimensional polyacrylamide gel electrophoresis revealed that three Wx loci, Wx-A1, WX-B1 and Wx-D1, located in chromosome arms 7AS, 4AL and 7DS produce three distinct Wx proteins, Wx-A1, Wx-B1 and Wx-D1 respectively. Wx-A1 possess higher molecular weight and more basic isoelectric-point (pI) than the other two. Wx-B1 and Wx-D1 possess the same molecular weight but a slightly different pI range. Owing to the detection of these three Wx proteins, we were able to identify the expression of three Wx genes in wheat endosperms and to find three types of mutants among Japanese wheat cultivars, first type was lacking for Wx-A1, second type lacking for Wx-B1 and third type lacking for both Wx-A1 and Wx-B1. These results suggested the possibility of breeding a waxy wheat.