(go
to KOMUGI Home) (go
to WIS List) (go
to NO.75 Contents)
Wheat Information
Service
Number 75: 41-45 (1992)
Inheritance
of resistance to leaf rust (Puccinia
recondita
f.
sp. tritici ) in two durum wheats
Sanjiv Gupta1, A. K. Gupta2 and R. G.
Saini3
Department of Genetics, Punjab Agricultural University, Ludhiana-141
004, Punjab, India
Summary
Inheritance studies were conducted on two leaf rust resistant durum
wheats. Triticum durum cvs. Malvika and CPAN 6051 were crossed
with susceptible durum wheat Malvi Local. The F2 and
F3 generations were tested against leaf rust races 1, 77
(biotype A) and 108. Single dominant gene was effective against race
108 and two independently inherited dominant genes were effective
against each of the races 1 and 77A in cultivar Malvika. Cultivar
CPAN 6051 carried single dominant gene against races 1 and 108,
whereas two independently inherited dominant genes were operative
against race 77A. At least one resistance gene was common in
cultivars Malvika and CPAN 6051.
Introduction
Breeding resistant cultivars depends primarily upon the availability
of diverse and effective resistance genes. Sources of resistance to
leaf rust caused by Puccinia recondita f. sp. tritici
have been identified both from Triticum aestivum (Gras
1980, Zitelli et al 1981, Tomerlin et al 1984, Dyck et al 1987) and
Triticum durum (Leonari 1973, Mishra et al. 1989, Casulli et
al 1983). Durum wheats have been identified as donors of new leaf
rust resistance (Lr) genes which are effective against highly
virulent races like 77 and 104 prevalent in the Indian sub-continent
(Sharma et al. 1986). Durum wheats also contribute towards superior
quality characteristics (Quick and Crawfod 1983). Identification of
Lr genes in Triticum aestivum is based on allelic tests
with near isogenic lines developed in the background of cultivars
Thatcher and Prelude (Dyck and Samborski 1970, Samborski and Dyck
1976, Dyck 1977). Such testers with designated genes are not
available for durums, therefore, only temporary designations have
been given to Lr genes identified from T. durum
(Ataullah 1969, Statler 1972). The present report deals with the
inheritance of resistance and allelic relationship between Lr
genes from two important durum wheats.
Material and methods
The experimental material comprised of two highly resistant durum
wheats namely Malvika and CPAN 6051, and a susceptible cultivar Malvi
Local. The pedigree, seedling reactions against race 1, 77 (biotype
A) and 108 and the field scores of these wheats in an epiphytotic of
race 77A are given in Table
1. Both resistant
wheats were crossed to the susceptible cultivar Malvi Local. The
F2 and F3 generations obtained from these
crosses were tested against leaf rust races 1, 77A and 108 at
seedling stage under controlled conditions in a growth house
maintained at 20 plus or minus 1oC. Simple
chi2-test was applied to test the goodness of fit of
different genetic ratios. Race1 is avirulent on all the Lr
genes from T. aestivum except Lr20 and Lr23
from T. durum both on seedlings and adult plants. At
seedling stage race 77A is virulent on all the Lr genes from
T. aestivum, but the adult plants carrying genes
Lr12, Lr14b, Lr23 and Lr27 + Lr31 from Triticum
aestivum are resistant. Race 108 is also virulent on all the
Lr genes from T. aestivum except Lr3 and Lr15
at seedling stage. The adult plants carrying Lr3, Lr12, Lr13,
Lr14a, Lr14b, Lr16, Lr17, Lr22, Lr22b, Lr23, Lr27 + Lr31
and Lr30 are resistant to this race.
The two resistant durums were also intercrossed and the F2
generation seedlings thus obtained were studied against races 1, 77A
and 108 to establish allelic relationship between genes from these
two wheats.
The infection types were recorded 14 days after inocultaion of seven
day old seedlings according to a modification of the scale given by
Stakman et al (1962).
1 Biotechnology
Centre, Punjab Agricultural University,
2 C. S. 1.
R. Complex, Palampur, Himachal Pradesh, India,
3 Corresponding
author
-->Next
(go
to KOMUGI Home) (go
to WIS List) (go
to NO.75 Contents)