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Wheat Information Service
Number 71: 14-15 (1990)


Association of different traits with monosomic addition lines of Aegilops squarrosa in Triticum durum

H. S. Dhaliwal, S. K. Sharma, S. Gupta and S. S. Bains

Punjab Agricultural University, Regional Research Station, Gurdaspur, Punjab, India


Certain alien-addition lines of Aegilops squarrosa in durum wheats have been used to study the association of certain characteristics with different D-genome chromosomes (Alston 1970; Makino 1981). However, in those attempts, it was not possible to recover and unequivocally identify the seven D-genome addition lines. Moreover, the available D-genome chromosome addition lines were developed in mixed backgrounds involving several durum parents. Dhaliwal et al (1990) for the first time developed a complete set of D-genome monosomic addition lines of Aegilops squarrosa (DD) accession 3754 in T. durum Desf. cv. PBW-114 using Giemsa C-banding. Four monosomic addition lines were for normal chromosomes 1D, 2D, 3D and 6D while the remaining three chromosomes 4D, 5D and 7D were represented by translocated chromosomes 4DS-5DS, 7DS-5DL and 7DL-4DL.

All the seven monosomic addition lines of Ae. squarrosa in durum background PBW-114 were planted at the Punjab Agricultural University, Regional Research Station, Gurdaspur, Punjab, India in winter 1989-90 for the investigation of association of various traits with individual D-genome chromosome. Monosomic addition plants for each of the addition lines were identified through cytological analysis and association of morphological characters (Dhaliwal et al 1990). Disease severity and other traits was recorded on five monosomic addition plants of the seven monosomic addition lines.

Monosomic addition lines 2D and 7DS-5DL possessed gene(s) for leaf rust and stripe rust susceptibility, respectively (
Table 1). Monosomic addition lines 3D and 6D were completely free from Karnal bunt, showing that these chromosomes carried epistatic gene(s) for Karnal bunt resistance. Gill et al (1987) also reported genes for Karnal bunt resistance on 3D and 6D chromosomes of T. aestivum by using ditelosomic series of T. aestivum cv. Chinese Spring. Chromosome 2D addition line had vitreous grains while all other lines and the durun parent had grains with yellow berry. Therefore, chromosome 2D, of Ae. squarrosa, possesses gene(s) for grain vitreousness. Gene(s) for round-shaped grain were present on chromosome 6D. Gene(s) for small grain size seemed to be located on chromosomes 1D and 3D as revealed by data on 1000-grain weight. Line 3D had the narrow leaves as compared to other addition lines. Makino (1981) also had reported genes for narrow leaves on chromosome 3D of Ae. squarrosa.

By using these seven monosomic addition lines Dhaliwal et al (1990) had reported the association of gene(s) for non-waxy character on chromosome 2D, lax head shape on chromosomes 2D, 7DS-5DL and 4DL-7DL, gene(s) for red glume colour on chromosome 1D, and red seed colour on chromosome 3D. The knowledge of association of gene(s) controlling disease resistance, seed characteristics, yield components and morphological traits with various Ae. squarrosa chromosomes can be used to identify the monosomic addition lines without resorting to cytological analysis, explore Ae. squarrosa germplasm for variability for the traits and breed durum and bread wheats using squarrosa lines with requisite variability.


References

Alston FH (1970) The addition of individual chromosomes of Aegilops squarrosa in Triticum durum. Cytologia 35: 402-408.

Makino T (1981) Cytogenetic, studies on the alien chromosome addition to durum wheat. Bull Natl Agric Exp St. 65: 1-58.

Dhaliwal HS, Friebe. B, Gill KS and Gill BS (1990) Cytogenetic identification of Aegilops squarrosa chromosome additions in durum wheat. Theor Appl Genet 79: 769-774.

Gill KS, Chand K, Dhaliwal HS, Nanda GS and Multani DS (1987) Karnal bunt studies on aneuploids of T. aestivum var. Chinese spring Ann Wheat Newsletter 33: 61-64.

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