| Chlorophyll analyses in X-ray induced mutants in Triticum
monococcum By Kosuke YAMASHITA Biol. Lab., Kyoto Univ., Kyoto A considerable number of chlorophyll mutants have been reported by SMITH (1939 and elsewhere), KIHARA and YAMASHITA (1947 and elsewhere), YAMASHITA (1953 and elsewhere) and FUJII (1955). These induced mutants have been proved to be Mendelian recessives due to single gene differences. Leaves taken from mature plants of some of these mutants were macerated in aceton with a small quantity of silicate powder and the extracts were submitted to paperchromatographic analyses of chlorophyll a and b. As the solvent, the upper layer of the mixture of petroleum ether, aceton and water in a ratio of 4: 1: 1 was used. As summarized in Table 1, chlorophyll b, is missing in yg mutant. In order to determine the ratio of chlorophyll a and b in these materials, a method of absorption spectra was adopted for the normals and the yellow-green. The chlorophyll extracts were obtained by grinding leaf cuts in chloroform with a small quantity of silicate powder. It is already known that chlorophyl1 a shows the maximum peak at 432 mmicro and 663 mmicro, while chlorophyll b at 456 mmicro and 644 mmicro. In advance, model absorption spectra of the mixture of chlorophyll a and b in certain ratios were prepared for comparison. The normals showed a similar. curve to that of the model in which chlorophyll a and b were mixed in a ratio of 1:1. In the yellow-green, no peak for chlorophyll b was found and a minor peak appeared at 480 mmicro and 630 mmicro. Further studies are now in progress. |