論文 - 詳細
| RRC ID | 66045 |
|---|---|
| 著者 | Takano W, Shibata S, Hagen N, Matsuda M, Otani Y. |
| タイトル | Minimizing scattering-induced phase errors in differential interference contrast microscopy. |
| ジャーナル | J Biomed Opt |
| Abstract |
SIGNIFICANCE:Differential interference contrast (DIC) microscopes allow noninvasive in vivo observation of transparent microstructures in tissue without the use of fluorescent dyes or genetic modification. We show how to modify a DIC microscope to measure the sample phase distribution accurately and in real-time even deep inside sample tissue. AIM:Our aim is to improve the DIC microscope's phase measurement to remove the phase bias that occurs in the presence of strong scattering. APPROACH:A quarter-wave plate was added in front of the polarization camera, allowing a modified phase calculation to incorporate all four polarization orientation angles (0 deg, 45 deg, 90 deg, and 135 deg) captured simultaneously by the polarization camera, followed by deconvolution. RESULTS:We confirm that the proposed method reduces phase measurement error in the presence of scattering and demonstrate the method using in vivo imaging of a beating heart inside a medaka egg and the whole-body blood circulation in a young medaka fish. CONCLUSIONS:Modifying a polarization-camera DIC microscope with a quarter-wave plate allows users to image deep inside samples without phase bias due to scattering effects. |
| 巻・号 | 25(12) |
| 公開日 | 2020-12-1 |
| DOI | 10.1117/1.JBO.25.12.123703 |
| PII | JBO-200280SSRR |
| PMID | 33319525 |
| PMC | PMC7734411 |
| MeSH | Animals Microscopy* Microscopy, Interference |
| IF | 2.785 |
| リソース情報 | |
| メダカ | IB178 HdrR-II1 MT827 T5 |