| RRC ID |
6432
|
| 著者 |
Penkner AM, Fridkin A, Gloggnitzer J, Baudrimont A, Machacek T, Woglar A, Csaszar E, Pasierbek P, Ammerer G, Gruenbaum Y, Jantsch V.
|
| タイトル |
Meiotic chromosome homology search involves modifications of the nuclear envelope protein Matefin/SUN-1.
|
| ジャーナル |
Cell
|
| Abstract |
Genome haploidization during meiosis depends on recognition and association of parental homologous chromosomes. The C. elegans SUN/KASH domain proteins Matefin/SUN-1 and ZYG-12 have a conserved role in this process. They bridge the nuclear envelope, connecting the cytoplasm and the nucleoplasm to transmit forces that allow chromosome movement and homolog pairing and prevent nonhomologous synapsis. Here, we show that Matefin/SUN-1 forms rapidly moving aggregates at putative chromosomal attachment sites in the meiotic transition zone (TZ). We analyzed requirements for aggregate formation and identified multiple phosphotarget residues in the nucleoplasmic domain of Matefin/SUN-1. These CHK-2 dependent phosphorylations occur in leptotene/zygotene, diminish during pachytene and are involved in pairing. Mimicking phosphorylation causes an extended TZ and univalents at diakinesis. Our data suggest that the properties of the nuclear envelope are altered during the time window when homologs are sorted and Matefin/SUN-1 aggregates form, thereby controling the movement, homologous pairing and interhomolog recombination of chromosomes.
|
| 巻・号 |
139(5)
|
| ページ |
920-33
|
| 公開日 |
2009-11-25
|
| DOI |
10.1016/j.cell.2009.10.045
|
| PII |
S0092-8674(09)01371-3
|
| PMID |
19913286
|
| MeSH |
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Checkpoint Kinase 2
Chromosome Pairing*
Chromosomes / metabolism
Meiosis*
Meiotic Prophase I
Microtubules / metabolism*
Mutation
Nuclear Envelope / metabolism
Phosphorylation
Protein Kinases / metabolism
Protein Structure, Tertiary
Receptors, Cytoplasmic and Nuclear / chemistry
Receptors, Cytoplasmic and Nuclear / metabolism*
Serine / metabolism
|
| IF |
38.637
|
| 引用数 |
123
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm853 |
