RRC ID 6420
著者 Ortiz CO, Faumont S, Takayama J, Ahmed HK, Goldsmith AD, Pocock R, McCormick KE, Kunimoto H, Iino Y, Lockery S, Hobert O.
タイトル Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases.
ジャーナル Curr Biol
Abstract BACKGROUND:Even though functional lateralization is a common feature of many nervous systems, it is poorly understood how lateralized neural function is linked to lateralized gene activity. A bilaterally symmetric pair of C. elegans gustatory neurons, ASEL and ASER, senses a number of chemicals in a left/right asymmetric manner and therefore serves as a model to study the genetic basis of functional lateralization. The extent of functional lateralization of the ASE neurons and genes responsible for the left/right asymmetric activity of ASEL and ASER is unknown.
RESULTS:We show here that a substantial number of salt ions are sensed in a left/right asymmetric manner and that lateralized salt responses allow the worm to discriminate between distinct salt cues. To identify molecules that may be involved in sensing salt ions and/or transmitting such sensory information, we examined the chemotaxis behavior of animals harboring mutations in eight different receptor-type, transmembrane guanylyl cyclases (encoded by gcy genes), which are expressed in either ASEL (gcy-6, gcy-7, gcy-14), ASER (gcy-1, gcy-4, gcy-5, gcy-22), or ASEL and ASER (gcy-19). Disruption of a particular ASER-expressed gcy gene, gcy-22, results in a broad chemotaxis defect to nearly all salts sensed by ASER, as well as to a left/right asymmetrically sensed amino acid. In contrast, disruption of other gcy genes resulted in highly salt ion-specific chemosensory defects.
CONCLUSIONS:Our findings broaden our understanding of lateralities in neural function, provide insights into how this laterality is molecularly encoded, and reveal an unusual multitude of molecules involved in gustatory signal transduction.
巻・号 19(12)
ページ 996-1004
公開日 2009-6-23
DOI 10.1016/j.cub.2009.05.043
PII S0960-9822(09)01178-6
PMID 19523832
PMC PMC2730525
MeSH Animals Behavior, Animal / physiology* Caenorhabditis elegans / anatomy & histology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chemotaxis / physiology Functional Laterality / physiology* Guanylate Cyclase / genetics Guanylate Cyclase / metabolism* Ions / chemistry Mutation Neurons / cytology Neurons / physiology Receptors, Guanylate Cyclase-Coupled / genetics Receptors, Guanylate Cyclase-Coupled / metabolism Salts / chemistry Taste / physiology*
IF 9.601
引用数 58
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm2364 tm2669