RRC ID 6373
著者 Hapiak VM, Hobson RJ, Hughes L, Smith K, Harris G, Condon C, Komuniecki P, Komuniecki RW.
タイトル Dual excitatory and inhibitory serotonergic inputs modulate egg laying in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Serotonin (5-HT) regulates key processes in both vertebrates and invertebrates. Previously, four 5-HT receptors that contributed to the 5-HT modulation of egg laying were identified in Caenorhabditis elegans. Therefore, to assess potential receptor interactions, we generated animals containing combinations of null alleles for each receptor, especially animals expressing only individual 5-HT receptors. 5-HT-stimulated egg laying and egg retention correlated well with different combinations of predicted excitatory and inhibitory serotonergic inputs. For example, 5-HT did not stimulate egg laying in ser-1, ser-7, or ser-7 ser-1 null animals, and ser-7 ser-1 animals retained more eggs than wild-type animals. In contrast, 5-HT-stimulated egg laying in ser-4;mod-1 animals was greater than in wild-type animals, and ser-4;mod-1 animals retained fewer eggs than wild-type animals. Surprisingly, ser-4;mod-1;ser-7 ser-1 animals retained the same number of eggs as wild-type animals and exhibited significant 5-HT-stimulated egg laying that was dependent on a previously uncharacterized receptor, SER-5. 5-HT-stimulated egg laying was absent in ser-5;ser-4;mod-1;ser-7 ser-1 animals, and these animals retained more eggs than either wild-type or ser-4;mod-1;ser-7 ser-1 animals. The 5-HT sensitivity of egg laying could be restored by ser-5 muscle expression. Together, these results highlight the dual excitatory/inhibitory serotonergic inputs that combine to modulate egg laying.
巻・号 181(1)
ページ 153-63
公開日 2009-1-1
DOI 10.1534/genetics.108.096891
PII genetics.108.096891
PMID 19001289
PMC PMC2621164
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Female Locomotion / drug effects Models, Biological Molecular Sequence Data Muscles / drug effects Muscles / metabolism Mutation / genetics Oviposition / drug effects Oviposition / physiology* Phylogeny Receptors, Serotonin / chemistry Serotonin / metabolism* Serotonin / pharmacology Signal Transduction* / drug effects
IF 4.015
引用数 35
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1325 tm1728 tm2654 tm2647 tm2564?