論文 - 詳細
| RRC ID | 6342 |
|---|---|
| 著者 | Cheng KC, Klancer R, Singson A, Seydoux G. |
| タイトル | Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition. |
| ジャーナル | Cell |
| Abstract |
DYRKs are kinases that self-activate in vitro by autophosphorylation of a YTY motif in the kinase domain, but their regulation in vivo is not well understood. In C. elegans zygotes, MBK-2/DYRK phosphorylates oocyte proteins at the end of the meiotic divisions to promote the oocyte-to-embryo transition. Here we demonstrate that MBK-2 is under both positive and negative regulation during the transition. MBK-2 is activated during oocyte maturation by CDK-1-dependent phosphorylation of serine 68, a residue outside of the kinase domain required for full activity in vivo. The pseudotyrosine phosphatases EGG-4 and EGG-5 sequester activated MBK-2 until the meiotic divisions by binding to the YTY motif and inhibiting MBK-2's kinase activity directly, using a mixed-inhibition mechanism that does not involve tyrosine dephosphorylation. Our findings link cell-cycle progression to MBK-2/DYRK activation and the oocyte-to-embryo transition. |
| 巻・号 | 139(3) |
| ページ | 560-72 |
| 公開日 | 2009-10-30 |
| DOI | 10.1016/j.cell.2009.08.047 |
| PII | S0092-8674(09)01187-8 |
| PMID | 19879842 |
| PMC | PMC2790168 |
| MeSH | Animals CDC2 Protein Kinase / metabolism* Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Embryo, Nonmammalian / metabolism Humans Oocytes / metabolism Protein-Tyrosine Kinases / metabolism* |
| IF | 38.637 |
| 引用数 | 65 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1191 |