論文 - 詳細
| RRC ID | 6317 |
|---|---|
| 著者 | Bae YK, Kim E, L'hernault SW, Barr MM. |
| タイトル | The CIL-1 PI 5-phosphatase localizes TRP Polycystins to cilia and activates sperm in C. elegans. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:C. elegans male sexual behaviors include chemotaxis and response to hermaphrodites, backing, turning, vulva location, spicule insertion, and sperm transfer, culminating in cross-fertilization of hermaphrodite oocytes with male sperm. The LOV-1 and PKD-2 transient receptor potential polycystin (TRPP) complex localizes to ciliated endings of C. elegans male-specific sensory neurons and mediates several aspects of male mating behavior. TRPP complex ciliary localization and sensory function are evolutionarily conserved. A genetic screen for C. elegans mutants with PKD-2 ciliary localization (Cil) defects led to the isolation of a mutation in the cil-1 gene. RESULTS:Here, we report that a phosphoinositide (PI) 5-phosphatase, CIL-1, regulates TRPP complex ciliary receptor localization and sperm activation. cil-1 does not regulate the localization of other ciliary proteins, including intraflagellar transport (IFT) components, sensory receptors, or other TRP channels in different cell types. Rather, cil-1 specifically controls TRPP complex trafficking in male-specific sensory neurons and does so in a cell-autonomous fashion. In these cells, cil-1 is required for normal PI(3)P distribution, indicating that a balance between PI(3,5)P2 and PI(3)P is important for TRPP localization. cil-1 mutants are infertile because of sperm activation and motility defects. In sperm, the CIL-1 5-phosphatase and a wortmannin-sensitive PI 3-kinase act antagonistically to regulate the conversion of sessile spermatids into motile spermatozoa, implicating PI(3,4,5)P3 signaling in nematode sperm activation. CONCLUSION:Our studies identify the CIL-1 5-phosphatase as a key regulator of PI metabolism in cell types that are important in several aspects of male reproductive biology. |
| 巻・号 | 19(19) |
| ページ | 1599-607 |
| 公開日 | 2009-10-13 |
| DOI | 10.1016/j.cub.2009.08.045 |
| PII | S0960-9822(09)01621-2 |
| PMID | 19781942 |
| PMC | PMC2762383 |
| MeSH | Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cluster Analysis DNA Primers / genetics Immunohistochemistry Male Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Phylogeny Sequence Analysis, DNA Sexual Behavior, Animal / physiology* Spermatozoa / metabolism Spermatozoa / physiology* TRPP Cation Channels / metabolism* Transient Receptor Potential Channels / metabolism* |
| IF | 9.601 |
| 引用数 | 39 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm2360 |