RRC ID 60950
著者 Vuong LT, Won JH, Nguyen MB, Choi KW.
タイトル Role of Armadillo repeat 2 and kinesin-II motor subunit Klp64D for wingless signaling in Drosophila.
ジャーナル Sci Rep
Abstract Armadillo (Arm) is crucial for transducing Wingless (Wg) signaling. Previously, we have shown that Klp64D, a motor subunit of Drosophila kinesin-II, interacts with Arm for Wg signaling. Molecular basis for this interaction has remained unknown. Here we identify a critical Arm repeat (AR) required for binding Klp64D and Wg signaling. Arm/[Formula: see text]-catenin family proteins contain a conserved domain of 12 Arm repeats (ARs). Five of these ARs can interact with Klp64D, but only the second AR (AR2) binds to the cargo/tail domain of Klp64D. Overexpression of AR2 in wing imaginal disc is sufficient to cause notched wing margin. This phenotype by AR2 is enhanced or suppressed by reducing or increasing Klp64D expression, respectively. AR2 overexpression inhibits Wg signaling activity in TopFlash assay, consistent with its dominant-negative effects on Klp64D-dependent Wg signaling. Overexpression of the Klp64D cargo domain also results in dominant-negative wing notching. Genetic rescue data indicate that both AR2 and Klp64D cargo regions are required for the function of Arm and Klp64D, respectively. AR2 overexpression leads to an accumulation of Arm with GM130 Golgi marker in Klp64D knockdown. This study suggests that Wg signaling for wing development is regulated by specific interaction between AR2 and the cargo domain of Klp64D.
巻・号 10(1)
ページ 13864
公開日 2020-8-17
DOI 10.1038/s41598-020-70759-8
PII 10.1038/s41598-020-70759-8
PMID 32807823
PMC PMC7431425
MeSH Animals Armadillo Domain Proteins / genetics* Armadillo Domain Proteins / physiology* Drosophila / genetics Drosophila / growth & development* Drosophila Proteins / genetics* Drosophila Proteins / physiology* Gene Expression Regulation, Developmental* Genes, Insect Kinesins / genetics* Kinesins / physiology Transcription Factors / genetics* Transcription Factors / physiology* Wings, Animal / growth & development* Wnt Signaling Pathway Wnt1 Protein / genetics*
IF 3.998
リソース情報
ショウジョウバエ 10642R-1 10642R-2