RRC ID 59632
著者 Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S.
タイトル Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation.
ジャーナル Cell Rep
Abstract piRNAs play significant roles in suppressing transposons and nonself nucleic acids, maintaining genome integrity, and defending against viral infections. In C. elegans, piRNA precursors are transcribed in the nucleus and are subjected to a number of processing and maturation steps. The biogenesis of piRNAs is not fully understood. We use functional proteomics in C. elegans and identify a piRNA biogenesis and chromosome segregation (PICS) complex. The PICS complex contains TOFU-6, PID-1, PICS-1, TOST-1, and ERH-2, which exhibit dynamic localization among different subcellular compartments. In the germlines, the PICS complex contains TOFU-6/PICS-1/ERH-2/PID-1, is largely concentrated at the perinuclear granule zone, and engages in piRNA processing. During embryogenesis, the TOFU-6/PICS-1/ERH-2/TOST-1 complex accumulates in the nucleus and plays essential roles in chromosome segregation. The functions of these factors in mediating chromosome segregation are independent of piRNA production. We speculate that differential compositions of PICS factors may help cells coordinate distinct cellular processes.
巻・号 27(12)
ページ 3561-3572.e3
公開日 2019-6-18
DOI 10.1016/j.celrep.2019.05.076
PII S2211-1247(19)30705-3
PMID 31216475
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Segregation* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Gene Expression Regulation* Germ Cells / cytology Germ Cells / metabolism Proteome / analysis* RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics*
IF 7.815
引用数 1
リソース情報
線虫 tm6709 tm6157 tm869 tm6424 tm2417