| RRC ID |
59532
|
| 著者 |
Lee J, Taylor CA, Barnes KM, Shen A, Stewart EV, Chen A, Xiang YK, Bao Z, Shen K.
|
| タイトル |
A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes.
|
| ジャーナル |
Elife
|
| Abstract |
Cellular differentiation requires both activation of target cell transcriptional programs and repression of non-target cell programs. The Myt1 family of zinc finger transcription factors contributes to fibroblast to neuron reprogramming in vitro. Here, we show that ztf-11 (Zinc-finger Transcription Factor-11), the sole Caenorhabditis elegans Myt1 homolog, is required for neurogenesis in multiple neuronal lineages from previously differentiated epithelial cells, including a neuron generated by a developmental epithelial-to-neuronal transdifferentiation event. ztf-11 is exclusively expressed in all neuronal precursors with remarkable specificity at single-cell resolution. Loss of ztf-11 leads to upregulation of non-neuronal genes and reduced neurogenesis. Ectopic expression of ztf-11 in epidermal lineages is sufficient to produce additional neurons. ZTF-11 functions together with the MuvB corepressor complex to suppress the activation of non-neuronal genes in neurons. These results dovetail with the ability of Myt1l (Myt1-like) to drive neuronal transdifferentiation in vitro in vertebrate systems. Together, we identified an evolutionarily conserved mechanism to specify neuronal cell fate by repressing non-neuronal genes.
|
| 巻・号 |
8
|
| 公開日 |
2019-8-6
|
| DOI |
10.7554/eLife.46703
|
| PII |
46703
|
| PMID |
31386623
|
| PMC |
PMC6684318
|
| MeSH |
Animals
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans Proteins / metabolism*
Cell Differentiation*
Epithelial Cells / physiology*
Gene Expression Regulation*
Neurons / physiology*
Trans-Activators / metabolism*
Transcription Factors
|
| IF |
7.551
|
| 引用数 |
2
|
| リソース情報 |
| 線虫 |
tm2315
tm5674
tm1768
tm4063
tm2061
tm1276 |
