| RRC ID |
59475
|
| 著者 |
Cuentas-Condori A, Mulcahy B, He S, Palumbos S, Zhen M, Miller DM 3rd.
|
| タイトル |
C. elegans neurons have functional dendritic spines.
|
| ジャーナル |
Elife
|
| Abstract |
Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook et al., 2018), suggesting that the experimental advantages of smaller model organisms could be exploited to study the biology of dendritic spines. Here, we used super-resolution microscopy, electron microscopy, live-cell imaging and genetics to show that C. elegans motor neurons have functional dendritic spines that: (1) are structurally defined by a dynamic actin cytoskeleton; (2) appose presynaptic dense projections; (3) localize ER and ribosomes; (4) display calcium transients triggered by presynaptic activity and propagated by internal Ca++ stores; (5) respond to activity-dependent signals that regulate spine density. These studies provide a solid foundation for a new experimental paradigm that exploits the power of C. elegans genetics and live-cell imaging for fundamental studies of dendritic spine morphogenesis and function.
|
| 巻・号 |
8
|
| 公開日 |
2019-10-4
|
| DOI |
10.7554/eLife.47918
|
| PII |
47918
|
| PMID |
31584430
|
| PMC |
PMC6802951
|
| MeSH |
Animals
Caenorhabditis elegans / cytology*
Dendritic Spines / ultrastructure*
Intravital Microscopy
Microscopy, Electron
Microscopy, Fluorescence
Motor Neurons / cytology*
Organelles / ultrastructure
|
| IF |
7.551
|
| 引用数 |
0
|
| リソース情報 |
| 線虫 |
tm2056 |
