論文 - 詳細
| RRC ID | 59451 |
|---|---|
| 著者 | Beurton F, Stempor P, Caron M, Appert A, Dong Y, Chen RA, Cluet D, Couté Y, Herbette M, Huang N, Polveche H, Spichty M, Bedet C, Ahringer J, Palladino F. |
| タイトル | Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin factors contribute to context dependent effects. Using a proteomics approach, we identified CFP1 associated proteins and an unexpected direct link between Caenorhabditis elegans CFP-1 and an Rpd3/Sin3 small (SIN3S) histone deacetylase complex. Supporting a functional connection, we find that mutants of COMPASS and SIN3 complex components genetically interact and have similar phenotypic defects including misregulation of common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 domain and recruits SIN-3 and the HDA-1/HDAC subunit to H3K4me3 enriched promoters. Our results reveal a novel role for CFP-1 in mediating interaction between SET1/COMPASS and a Sin3S HDAC complex at promoters. |
| 巻・号 | 47(21) |
| ページ | 11164-11180 |
| 公開日 | 2019-12-2 |
| DOI | 10.1093/nar/gkz880 |
| PII | 5585547 |
| PMID | 31602465 |
| PMC | PMC6868398 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Embryo, Nonmammalian Histone-Lysine N-Methyltransferase / metabolism* Histone-Lysine N-Methyltransferase / physiology Multiprotein Complexes / metabolism Multiprotein Complexes / physiology* Protein Binding Sin3 Histone Deacetylase and Corepressor Complex / metabolism* Trans-Activators / metabolism* |
| IF | 11.147 |
| 引用数 | 0 |
| リソース情報 | |
| 線虫 | tm1276 tm6369 tm4223 tm4302 |