論文 - 詳細
| RRC ID | 59412 |
|---|---|
| 著者 | Tang LT, Diaz-Balzac CA, Rahman M, Ramirez-Suarez NJ, Salzberg Y, Lázaro-Peña MI, Bülow HE. |
| タイトル | TIAM-1/GEF can shape somatosensory dendrites independently of its GEF activity by regulating F-actin localization. |
| ジャーナル | Elife |
| Abstract |
Dendritic arbors are crucial for nervous system assembly, but the intracellular mechanisms that govern their assembly remain incompletely understood. Here, we show that the dendrites of PVD neurons in Caenorhabditis elegans are patterned by distinct pathways downstream of the DMA-1 leucine-rich transmembrane (LRR-TM) receptor. DMA-1/LRR-TM interacts through a PDZ ligand motif with the guanine nucleotide exchange factor TIAM-1/GEF in a complex with act-4/Actin to pattern higher order 4° dendrite branches by localizing F-actin to the distal ends of developing dendrites. Surprisingly, TIAM-1/GEF appears to function independently of Rac1 guanine nucleotide exchange factor activity. A partially redundant pathway, dependent on HPO-30/Claudin, regulates formation of 2° and 3° branches, possibly by regulating membrane localization and trafficking of DMA-1/LRR-TM. Collectively, our experiments suggest that HPO-30/Claudin localizes the DMA-1/LRR-TM receptor on PVD dendrites, which in turn can control dendrite patterning by directly modulating F-actin dynamics through TIAM-1/GEF. |
| 巻・号 | 8 |
| 公開日 | 2019-1-29 |
| DOI | 10.7554/eLife.38949 |
| PII | 38949 |
| PMID | 30694177 |
| PMC | PMC6370339 |
| MeSH | Actins / genetics* Actins / metabolism Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Claudins / genetics Claudins / metabolism Gene Expression Regulation Membrane Proteins / genetics* Membrane Proteins / metabolism Nervous System / cytology Nervous System / growth & development Nervous System / metabolism Neurogenesis / genetics Neuronal Plasticity / genetics* Neurons / cytology Neurons / metabolism PDZ Domains Protein Binding Protein Transport Synaptic Transmission T-Lymphoma Invasion and Metastasis-inducing Protein 1 / genetics* T-Lymphoma Invasion and Metastasis-inducing Protein 1 / metabolism rac1 GTP-Binding Protein / genetics rac1 GTP-Binding Protein / metabolism |
| IF | 7.551 |
| 引用数 | 6 |
| リソース情報 | |
| 線虫 | tm1556 tm5159 |