| RRC ID |
58947
|
| 著者 |
Abe K, Shimada A, Tayama S, Nishikawa H, Kaneko T, Tsuda S, Karaiwa A, Matsui T, Ishitani T, Takeda H.
|
| タイトル |
Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Establishment of robust gene expression boundary is crucial for creating elaborate morphology during development. However, mechanisms underlying boundary formation have been extensively studied only in a few model systems. We examined the establishment of zic1/zic4-expression boundary demarcating dorsoventral boundary of the entire trunk of medaka fish (Oryzias latipes) and identified a subgroup of dermomyotomal cells called horizontal boundary cells (HBCs) as crucial players for the boundary formation. Embryological and genetic analyses demonstrated that HBCs play crucial roles in the two major events of the process, i.e., refinement and maintenance. In the refinement, HBCs could serve as a chemical barrier against Wnts from the neural tube by expressing Hhip. At later stages, HBCs participate in the maintenance of the boundary by differentiating into the horizontal myoseptum physically inhibiting cell mixing across the boundary. These findings reveal the mechanisms underlying the dorsoventral boundary in the teleost trunk by specialized boundary cells.
|
| 巻・号 |
27(3)
|
| ページ |
928-939.e4
|
| 公開日 |
2019-4-16
|
| DOI |
10.1016/j.celrep.2019.03.068
|
| PII |
S2211-1247(19)30398-5
|
| PMID |
30995487
|
| MeSH |
Animals
Animals, Genetically Modified / metabolism
Body Patterning / genetics
Cell Differentiation
Chromosomes, Artificial, Bacterial / genetics
Fish Proteins / genetics
Fish Proteins / metabolism*
Gene Expression Regulation, Developmental
Oryzias / metabolism
Somites / cytology
Somites / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism*
Zebrafish / metabolism
|
| IF |
8.109
|
| リソース情報 |
| メダカ |
d-rR/TOKYO (MT837)
d-rR-Tg(beta-actin-loxP-non-loxP-GFP) (TG891)
Saori (WS234) |
| ヒト・動物細胞 |
COS-7(RCB0539) |
