論文 - 詳細
| RRC ID | 5857 |
|---|---|
| 著者 | Inohaya K, Takano Y, Kudo A. |
| タイトル | Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. |
| ジャーナル | Development |
| Abstract |
The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused centrum (fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, we found that wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. The establishment of the transgenic rescue lines and mosaic analyses allowed the conclusion to be drawn that production of wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column. Our findings provide a novel perspective on the mechanism of vertebrate development. |
| 巻・号 | 137(11) |
| ページ | 1807-13 |
| 公開日 | 2010-6-1 |
| DOI | 10.1242/dev.051540 |
| PII | 137/11/1807 |
| PMID | 20460365 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Body Patterning / genetics Body Patterning / physiology Cloning, Molecular DNA Primers / genetics Fish Proteins / biosynthesis* Fish Proteins / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Male Molecular Sequence Data Mutation Neural Tube / cytology Neural Tube / embryology Neural Tube / metabolism Oryzias / embryology* Oryzias / genetics Oryzias / growth & development Oryzias / metabolism* Osteoblasts / cytology Osteoblasts / metabolism Phenotype Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spine / embryology* Spine / growth & development Spine / metabolism* Wnt Proteins / biosynthesis* Wnt Proteins / genetics |
| IF | 5.611 |
| 引用数 | 26 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| メダカ | medaka Hd-rR fosmid DNA Qurt (MT828) HNI-II (IB176) fu (MT51) Fused(BBRRfufu) (MT1122) wnt4b-EGFP (TG1259) wnt4b^fsc/fsc-Tg(wnt4b-zfcry:EGFP) (TG1260) |