RRC ID 5707
著者 Pfennig F, Kind B, Zieschang F, Busch M, Gutzeit HO.
タイトル Tert expression and telomerase activity in gonads and somatic cells of the Japanese medaka (Oryzias latipes).
ジャーナル Dev Growth Differ
Abstract A telomerase reverse transcriptase (Tert) encoding gene was cloned from the testis of the teleost fish Oryzias latipes. The expression pattern of Japanese medaka tert (Ola_tert) was analyzed by reverse transcription-polymerase chain reaction and in situ hybridization. Ola_tert was expressed in embryonic stages as well as in differentiated adult tissues. In tissues of adult medakas the highest tert expression was found in gonads and brain. Furthermore, two different splice variants were described and an Ola_tert antisense transcript was identified. The enzyme activity of Tert was determined using a non-radioactive telomeric amplification protocol and the telomerase activity in various tissues was shown to correlate with the tert expression. The telomerase activity was found to be high in contrast to the generally low activity in differentiated human tissues.
巻・号 50(3)
ページ 131-41
公開日 2008-3-1
DOI 10.1111/j.1440-169X.2008.00986.x
PII DGD986
PMID 18312429
MeSH Alternative Splicing Amino Acid Motifs Amino Acid Sequence Animals Brain / enzymology* Female Gene Expression Regulation, Enzymologic Isoenzymes / genetics Male Molecular Sequence Data Myocardium / enzymology Nerve Tissue Proteins / analysis Nerve Tissue Proteins / biosynthesis Nerve Tissue Proteins / genetics Organ Specificity Oryzias / embryology Oryzias / genetics Oryzias / growth & development Oryzias / metabolism* RNA, Antisense / analysis RNA, Antisense / genetics Sequence Alignment Sequence Homology, Amino Acid Telomerase / analysis Telomerase / biosynthesis* Telomerase / genetics Testis / enzymology*
IF 1.723
引用数 13
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
メダカ d-rR.YHNI FLFII (MT109)