RRC ID 5683
著者 Pant S, Sharma M, Patel K, Caplan S, Carr CM, Grant BD.
タイトル AMPH-1/Amphiphysin/Bin1 functions with RME-1/Ehd1 in endocytic recycling.
ジャーナル Nat Cell Biol
Abstract RME-1/EHD1 (receptor mediated endocytosis/Eps15 homology-domain containing 1) family proteins are key residents of the recycling endosome, which are required for endosome-to-plasma membrane transport in Caenorhabditis elegans and mammals. Recent studies suggest similarities between the RME-1/EHD proteins and the Dynamin GTPase superfamily of mechanochemical pinchases, which promote membrane fission. Here we show that endogenous C. elegans AMPH-1, the only C. elegans member of the Amphiphysin/BIN1 family of BAR (Bin1-Amphiphysin-Rvs161p/167p)-domain-containing proteins, colocalizes with RME-1 on recycling endosomes in vivo, that amph-1-deletion mutants are defective in recycling endosome morphology and function, and that binding of AMPH-1 Asn-Pro-Phe(Asp/Glu) sequences to the RME-1 EH-domain promotes the recycling of transmembrane cargo. We also show a requirement for human BIN1 (also known as Amphiphysin 2) in EHD1-regulated endocytic recycling. In vitro, we find that purified recombinant AMPH-1-RME-1 complexes produce short, coated membrane tubules that are qualitatively distinct from those produced by either protein alone. Our results indicate that AMPH-1 and RME-1 cooperatively regulate endocytic recycling, probably through functions required for the production of cargo carriers that exit the recycling endosome for the cell surface.
巻・号 11(12)
ページ 1399-410
公開日 2009-12-1
DOI 10.1038/ncb1986
PII ncb1986
PMID 19915558
PMC PMC2788952
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / ultrastructure Endocytosis* HeLa Cells Humans Microscopy, Electron Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Nerve Tissue Proteins / ultrastructure Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding RNA Interference Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism*
IF 20.042
引用数 120
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1060