| RRC ID |
55707
|
| 著者 |
Nagasawa K, Ishida M, Octavera A, Kusano K, Kezuka F, Kitano T, Yoshiura Y, Yoshizaki G.
|
| タイトル |
Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation†.
|
| ジャーナル |
Biol Reprod
|
| Abstract |
A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP). We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (-/-)) recovered their fertility and produced only donor-derived fshr (-) X eggs. Natural mating between these females and fshr (-/-) sex-reversed XX males successfully produced large numbers of sterile fshr (-/-) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established.
|
| 巻・号 |
100(2)
|
| ページ |
535-546
|
| 公開日 |
2019-2-1
|
| DOI |
10.1093/biolre/ioy204
|
| PII |
5106316
|
| PMID |
30252024
|
| MeSH |
Animals
Aromatase Inhibitors / pharmacology
Female
Gene Deletion
Gene Expression Regulation / drug effects
Gene Expression Regulation / genetics
Genotype
Hybridization, Genetic
Infertility / genetics
Infertility / veterinary*
Male
Oryzias / genetics*
Oryzias / physiology*
Receptors, FSH / genetics*
Spermatogonia / physiology*
|
| IF |
2.96
|
| 引用数 |
2
|
| リソース情報 |
| メダカ |
hi-medaka (MT835) |
