RRC ID 54494
著者 Sun Y, Zhang D, Li C, Huang J, Li W, Qiu Y, Mao A, Zhou M, Xue L.
タイトル Lic regulates JNK-mediated cell death in Drosophila.
ジャーナル Cell Prolif
Abstract OBJECTIVES:The evolutionary conserved JNK pathway plays crucial role in cell death, yet factors that modulate this signalling have not been fully disclosed. In this study, we aim to identify additional factors that regulate JNK signalling in cell death, and characterize the underlying mechanisms.
MATERIALS AND METHODS:Drosophila were raised on standard media, and cross was carried out at 25°C. The Gal4/UAS system was used to express proteins or RNAi in a specific temporal and spatial pattern. Gene expression was revealed by GFP fluorescence, X-gal staining or immunostaining of 3rd instar larval eye and wing discs. Cell death was visualized by acridine orange (AO) staining. Images of fly eyes and wings were taken by OLYMPUS microscopes.
RESULTS:We found that licorne (lic) encoding the Drosophila MKK3 is an essential regulator of JNK-mediated cell death. Firstly, loss of lic suppressed ectopic Egr-triggered JNK activation and cell death in eye and wing development. Secondary, lic is necessary for loss-of-cell polarity-induced, physiological JNK-dependent cell death in wing development. Thirdly, Lic overexpression is sufficient to initiate JNK-mediated cell death in developing eyes and wings. Furthermore, ectopic Lic activates JNK signalling by promoting JNK phosphorylation. Finally, genetic epistatic analysis confirmed that Lic acts in parallel with Hep in the Egr-JNK pathway.
CONCLUSIONS:This study not only identified Lic as a novel component of the JNK signalling, but also disclosed the crucial roles and mechanism of Lic in cell death.
巻・号 52(3)
ページ e12593
公開日 2019-5-1
DOI 10.1111/cpr.12593
PMID 30847993
PMC PMC6536442
MeSH Animals Animals, Genetically Modified Cell Death* / genetics Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Epistasis, Genetic Eye / growth & development Eye / metabolism Gene Expression Regulation, Developmental Genes, Insect MAP Kinase Signaling System* / genetics Membrane Proteins / genetics Mitogen-Activated Protein Kinase Kinases / genetics Protein Kinases / genetics Protein Kinases / metabolism* RNA Interference Wings, Animal / growth & development Wings, Animal / metabolism
IF 5.039
引用数 2
リソース情報
ショウジョウバエ 1388R-2 7393R-1