RRC ID 53954
著者 Shen EZ, Chen H, Ozturk AR, Tu S, Shirayama M, Tang W, Ding YH, Dai SY, Weng Z, Mello CC.
タイトル Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline.
ジャーナル Cell
Abstract piRNAs (Piwi-interacting small RNAs) engage Piwi Argonautes to silence transposons and promote fertility in animal germlines. Genetic and computational studies have suggested that C. elegans piRNAs tolerate mismatched pairing and in principle could target every transcript. Here we employ in vivo cross-linking to identify transcriptome-wide interactions between piRNAs and target RNAs. We show that piRNAs engage all germline mRNAs and that piRNA binding follows microRNA-like pairing rules. Targeting correlates better with binding energy than with piRNA abundance, suggesting that piRNA concentration does not limit targeting. In mRNAs silenced by piRNAs, secondary small RNAs accumulate at the center and ends of piRNA binding sites. In germline-expressed mRNAs, however, targeting by the CSR-1 Argonaute correlates with reduced piRNA binding density and suppression of piRNA-associated secondary small RNAs. Our findings reveal physiologically important and nuanced regulation of individual piRNA targets and provide evidence for a comprehensive post-transcriptional regulatory step in germline gene expression.
巻・号 172(5)
ページ 937-951.e18
公開日 2018-2-22
DOI 10.1016/j.cell.2018.02.002
PII S0092-8674(18)30117-X
PMID 29456082
PMC PMC5905434
MeSH Amino Acid Sequence Animals Base Pairing Base Sequence Binding Sites Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Chimera / metabolism Gene Silencing Germ Cells / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism*
IF 38.637
引用数 39
リソース情報
線虫 tm872