RRC ID |
53954
|
著者 |
Shen EZ, Chen H, Ozturk AR, Tu S, Shirayama M, Tang W, Ding YH, Dai SY, Weng Z, Mello CC.
|
タイトル |
Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline.
|
ジャーナル |
Cell
|
Abstract |
piRNAs (Piwi-interacting small RNAs) engage Piwi Argonautes to silence transposons and promote fertility in animal germlines. Genetic and computational studies have suggested that C. elegans piRNAs tolerate mismatched pairing and in principle could target every transcript. Here we employ in vivo cross-linking to identify transcriptome-wide interactions between piRNAs and target RNAs. We show that piRNAs engage all germline mRNAs and that piRNA binding follows microRNA-like pairing rules. Targeting correlates better with binding energy than with piRNA abundance, suggesting that piRNA concentration does not limit targeting. In mRNAs silenced by piRNAs, secondary small RNAs accumulate at the center and ends of piRNA binding sites. In germline-expressed mRNAs, however, targeting by the CSR-1 Argonaute correlates with reduced piRNA binding density and suppression of piRNA-associated secondary small RNAs. Our findings reveal physiologically important and nuanced regulation of individual piRNA targets and provide evidence for a comprehensive post-transcriptional regulatory step in germline gene expression.
|
巻・号 |
172(5)
|
ページ |
937-951.e18
|
公開日 |
2018-2-22
|
DOI |
10.1016/j.cell.2018.02.002
|
PII |
S0092-8674(18)30117-X
|
PMID |
29456082
|
PMC |
PMC5905434
|
MeSH |
Amino Acid Sequence
Animals
Base Pairing
Base Sequence
Binding Sites
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Chimera / metabolism
Gene Silencing
Germ Cells / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism*
|
IF |
38.637
|
引用数 |
39
|
リソース情報 |
線虫 |
tm872 |