| RRC ID |
53952
|
| 著者 |
Lloret-Fernández C, Maicas M, Mora-Martínez C, Artacho A, Jimeno-Martín Á, Chirivella L, Weinberg P, Flames N.
|
| タイトル |
A transcription factor collective defines the HSN serotonergic neuron regulatory landscape.
|
| ジャーナル |
Elife
|
| Abstract |
Cell differentiation is controlled by individual transcription factors (TFs) that together activate a selection of enhancers in specific cell types. How these combinations of TFs identify and activate their target sequences remains poorly understood. Here, we identify the cis-regulatory transcriptional code that controls the differentiation of serotonergic HSN neurons in Caenorhabditis elegans. Activation of the HSN transcriptome is directly orchestrated by a collective of six TFs. Binding site clusters for this TF collective form a regulatory signature that is sufficient for de novo identification of HSN neuron functional enhancers. Among C. elegans neurons, the HSN transcriptome most closely resembles that of mouse serotonergic neurons. Mouse orthologs of the HSN TF collective also regulate serotonergic differentiation and can functionally substitute for their worm counterparts which suggests deep homology. Our results identify rules governing the regulatory landscape of a critically important neuronal type in two species separated by over 700 million years.
|
| 巻・号 |
7
|
| 公開日 |
2018-3-22
|
| DOI |
10.7554/eLife.32785
|
| PII |
32785
|
| PMID |
29553368
|
| PMC |
PMC5916565
|
| MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Cell Differentiation / genetics
Gene Expression Profiling*
HEK293 Cells
Humans
Mice, Inbred C57BL
Phylogeny
Serotonergic Neurons / metabolism*
Transcription Factors / classification
Transcription Factors / genetics*
Transcription Factors / metabolism
|
| IF |
7.08
|
| 引用数 |
10
|
| リソース情報 |
| 線虫 |
tm1688 |
