| RRC ID |
53942
|
| 著者 |
Matsunaga Y, Matsukawa T, Iwasaki T, Nagata K, Kawano T.
|
| タイトル |
Comparison of physiological functions of antagonistic insulin-like peptides, INS-23 and INS-18, in Caenorhabditis elegans.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
In Caenorhabditis elgans, insulin-like peptides have significant roles in modulating larval diapause and adult lifespan via the insulin/IGF-1 signaling (IIS) pathway. Although 40 insulin-like peptides (ILPs) have been identified, it remains unknown how ILPs act as either agonists or antagonists for their sole receptor, DAF-2. Here we found 1) INS-23 functions as an antagonistic ILP to promote larval diapause through the IIS pathway like a DAF-2 antagonist, INS-18, 2) INS-23 and INS-18 have similar biochemical functions. In addition, our molecular modeling suggests that INS-23 and INS-18 have characteristic insertions in the B-domain, which are crucial for the recognition of the insulin receptor, when compared with DAF-2 agonists. These characteristic insertions in the B-domain of INS-23 and INS-18 would modulate their intermolecular interactions with the DAF-2 receptor, which may lead these molecules to act as antagonistic ligands. Our study provides new insight into the function and structure of ILPs.
|
| 巻・号 |
82(1)
|
| ページ |
90-96
|
| 公開日 |
2018-1-1
|
| DOI |
10.1080/09168451.2017.1415749
|
| PMID |
29303423
|
| MeSH |
Animals
Caenorhabditis elegans
Insulin-Like Growth Factor I / pharmacology*
Peptide Hormones / physiology*
Signal Transduction / drug effects
|
| IF |
1.516
|
| 引用数 |
4
|
| リソース情報 |
| 線虫 |
tm339
tm1875 |
