論文 - 詳細
| RRC ID | 53531 |
|---|---|
| 著者 | Nechipurenko IV, Olivier-Mason A, Kazatskaya A, Kennedy J, McLachlan IG, Heiman MG, Blacque OE, Sengupta P. |
| タイトル | A Conserved Role for Girdin in Basal Body Positioning and Ciliogenesis. |
| ジャーナル | Dev Cell |
| Abstract |
Primary cilia are ubiquitous sensory organelles that mediate diverse signaling pathways. Cilia position on the cell surface is determined by the location of the basal body (BB) that templates the cilium. The mechanisms that regulate BB positioning in the context of ciliogenesis are largely unknown. Here we show that the conserved signaling and scaffolding protein Girdin localizes to the proximal regions of centrioles and regulates BB positioning and ciliogenesis in Caenorhabditis elegans sensory neurons and human RPE-1 cells. Girdin depletion alters localization of the intercentriolar linker and ciliary rootlet component rootletin, and rootletin knockdown in RPE-1 cells mimics Girdin-dependent phenotypes. C. elegans Girdin also regulates localization of the apical junction component AJM-1, suggesting that in nematodes Girdin may position BBs via rootletin- and AJM-1-dependent anchoring to the cytoskeleton and plasma membrane, respectively. Together, our results describe a conserved role for Girdin in BB positioning and ciliogenesis. |
| 巻・号 | 38(5) |
| ページ | 493-506 |
| 公開日 | 2016-9-12 |
| DOI | 10.1016/j.devcel.2016.07.013 |
| PII | S1534-5807(16)30502-0 |
| PMID | 27623382 |
| PMC | PMC5023068 |
| MeSH | Animals Basal Bodies / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Centrioles / genetics Cilia / genetics Cilia / metabolism* Cytoskeletal Proteins / genetics* Cytoskeleton / genetics Humans Microfilament Proteins / biosynthesis Microfilament Proteins / genetics* Microtubules / genetics Morphogenesis / genetics* Organelles / genetics Sensory Receptor Cells / metabolism Signal Transduction / genetics Vesicular Transport Proteins / biosynthesis Vesicular Transport Proteins / genetics* |
| IF | 10.092 |
| 引用数 | 13 |
| リソース情報 | |
| 線虫 | tm6493 |