RRC ID 53471
著者 Tang W, Tu S, Lee HC, Weng Z, Mello CC.
タイトル The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans.
ジャーナル Cell
Abstract Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of piRNAs is that, while piRNA lengths are stereotypical within a species, they can differ widely between species. For example, piRNAs are mainly 29 and 30 nucleotides in humans, 24 to 30 nucleotides in D. melanogaster, and uniformly 21 nucleotides in C. elegans. However, how piRNA length is determined and whether length impacts function remains unknown. Here, we show that C. elegans deficient for PARN-1, a conserved RNase, accumulate untrimmed piRNAs with 3' extensions. Surprisingly, these longer piRNAs are stable and associate with the Piwi protein PRG-1 but fail to robustly recruit downstream silencing factors. Our findings identify PARN-1 as a key regulator of piRNA length in C. elegans and suggest that length is regulated to promote efficient transcriptome surveillance.
巻・号 164(5)
ページ 974-84
公開日 2016-2-25
DOI 10.1016/j.cell.2016.02.008
PII S0092-8674(16)30074-5
PMID 26919432
PMC PMC4785802
MeSH Amino Acid Sequence Animals Argonaute Proteins / metabolism Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Exoribonucleases / chemistry Exoribonucleases / metabolism* Metabolic Networks and Pathways Molecular Sequence Data Mutation RNA Processing, Post-Transcriptional* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Sequence Alignment Transcriptome
IF 38.637
引用数 52
リソース情報
線虫 tm872 tm1339 tm4477 tm869