論文 - 詳細
RRC ID | 53427 |
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著者 | Seo B, Kim C, Hills M, Sung S, Kim H, Kim E, Lim DS, Oh HS, Choi RMJ, Chun J, Shim J, Lee J. |
タイトル | Telomere maintenance through recruitment of internal genomic regions. |
ジャーナル | Nat Commun |
Abstract |
Cells surviving crisis are often tumorigenic and their telomeres are commonly maintained through the reactivation of telomerase. However, surviving cells occasionally activate a recombination-based mechanism called alternative lengthening of telomeres (ALT). Here we establish stably maintained survivors in telomerase-deleted Caenorhabditis elegans that escape from sterility by activating ALT. ALT survivors trans-duplicate an internal genomic region, which is already cis-duplicated to chromosome ends, across the telomeres of all chromosomes. These 'Template for ALT' (TALT) regions consist of a block of genomic DNA flanked by telomere-like sequences, and are different between two genetic background. We establish a model that an ancestral duplication of a donor TALT region to a proximal telomere region forms a genomic reservoir ready to be incorporated into telomeres on ALT activation. |
巻・号 | 6 |
ページ | 8189 |
公開日 | 2015-9-18 |
DOI | 10.1038/ncomms9189 |
PII | ncomms9189 |
PMID | 26382656 |
PMC | PMC4595603 |
MeSH | Animals Animals, Genetically Modified Blotting, Southern Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics* DNA / genetics* In Situ Hybridization, Fluorescence Recombination, Genetic / genetics* Telomerase / genetics* Telomere Homeostasis / genetics* |
IF | 12.121 |
引用数 | 9 |
リソース情報 | |
線虫 | tm1620 tm1400 |