RRC ID 53427
著者 Seo B, Kim C, Hills M, Sung S, Kim H, Kim E, Lim DS, Oh HS, Choi RMJ, Chun J, Shim J, Lee J.
タイトル Telomere maintenance through recruitment of internal genomic regions.
ジャーナル Nat Commun
Abstract Cells surviving crisis are often tumorigenic and their telomeres are commonly maintained through the reactivation of telomerase. However, surviving cells occasionally activate a recombination-based mechanism called alternative lengthening of telomeres (ALT). Here we establish stably maintained survivors in telomerase-deleted Caenorhabditis elegans that escape from sterility by activating ALT. ALT survivors trans-duplicate an internal genomic region, which is already cis-duplicated to chromosome ends, across the telomeres of all chromosomes. These 'Template for ALT' (TALT) regions consist of a block of genomic DNA flanked by telomere-like sequences, and are different between two genetic background. We establish a model that an ancestral duplication of a donor TALT region to a proximal telomere region forms a genomic reservoir ready to be incorporated into telomeres on ALT activation.
巻・号 6
ページ 8189
公開日 2015-9-18
DOI 10.1038/ncomms9189
PII ncomms9189
PMID 26382656
PMC PMC4595603
MeSH Animals Animals, Genetically Modified Blotting, Southern Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics* DNA / genetics* In Situ Hybridization, Fluorescence Recombination, Genetic / genetics* Telomerase / genetics* Telomere Homeostasis / genetics*
IF 12.121
引用数 9
リソース情報
線虫 tm1620 tm1400